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1.14.14.86: ent-kaurene monooxygenase

This is an abbreviated version!
For detailed information about ent-kaurene monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.86

Reaction

ent-kaur-16-en-19-al
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
ent-kaur-16-en-19-oate
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

AtKO, CYP701, CYP701A, CYP701A26, CYP701A3, CYP701A50, CYP701A51, CYP701A6, CYP701B1, EC 1.14.13.78, EKO, ent-kaurene oxidase, ent-kaurene oxidase 2, ent-kaurene oxidase-like protein, kaurene oxidase, KO, KO1, KO2, KOS1, KOS2, KOS3, KOS4, More, MtKO, OsKO, OsKO2, P450-4, PsKO1, SmKO, TwKO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.86 ent-kaurene monooxygenase

Cloned

Cloned on EC 1.14.14.86 - ent-kaurene monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned from leaf microsomes, functional recombinant expression in Saccharomyces cerevisiae
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cloning of cDNA
cloning of the enzyme from hairy roots of Salvia miltiorrhiza, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, SmKO is co-expressed with the fused enzyme SmKS-SmCPSent and a NADPH-cytochrome P450 reductase (SmCPR1) in the yeast strain SGH5
CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
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CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification
DNA and amino acid sequence determimnation and analysis, expression in Escherichia coli, Saccharomyces cerevisiae, and in transgenic Arabidopsis thaliana leaves as GFP-tagged protein via Agrobacterium tumefaciens transfection, expression of the GFP-tagged enzyme in onion bulb epidermis or tobacco seedling leaves using particle bombardment method
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DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV7, Q68YV9, Q6GZ42
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Pichia pastoris
expression in Escherichia coli
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression of cDNA in Saccharomyces cerevisiae
-
gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
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gene CYP701A3, phylogenetic analysis
gene CYP701A6, phylogenetic analysis
gene CYP701B1, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris microsomes
gene LsKO1, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
gene LsKO2, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
gene P450-4 from strain D02945, DNA and amino acid sequence determination and analysis, organization of the GA gene cluster, expression in Escherichia coli and in strain D02945, screening for ectopic integrations, P450-4 promoter studies using the GUS reporter system, expression analysis
KOS2, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV7, Q68YV9, Q6GZ42
KOS3, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV8
KOS4, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV7, Q68YV9, Q6GZ42
overexpression in Arabidopsis thaliana
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PCR-amplification of cDNA, cloning into pPICZ yeast expression vector, expression in Pichia pastoris strain X-33 together with fungal P450 reductase (CPR)
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phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant ent-kaurene oxidase expression in Saccharomyces cerevisiae strain BY-T20, coexpression with Tripterygium wilfordii CPR1-4 genes encoding four cytochrome P450 reductases, recombinant expression of enzyme mutants
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