Cloned (Comment) | Organism |
---|---|
gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme modification by N-terminal deletion of the first 43 or 40 amino acids and replacing with 10 amino acid lysine-rich sequences of MAKKTSSKGK | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | - |
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | - |
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Zea mays | - |
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Zea mays | overall reaction | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | - |
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Zea mays | CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | CYP701A26 shows constitutive expression in active growing tissues and no inducible expression | Zea mays | - |
seedling | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Zea mays | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Zea mays | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
isokaurene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid | Zea mays | ? | - |
? | |
additional information | CYP701A26 exhibits substrate promiscuity to catalyze oxidation of other labdane related diterpenes | Zea mays | ? | - |
? | |
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | 3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP701A26 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Zea mays | |
NADPH | - |
Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | constitutive expression in active growing tissues and no inducible expression | additional information |
Zea mays | CYP701A26 shows no inducible expression | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belong to the CYP701A subfamily | Zea mays |
metabolism | oxidation of ent-kaurene to ent-kaurenoic acid is involved in gibberellin biosynthesis | Zea mays |
physiological function | the enzyme is involved in gibberellin biosynthesis and catalyzes carboxylation on C19 of ent-kaurene to generate ent-kaurenoic acid in plastids. The tetracyclic diterpenoid plant hormones, gibberellins, play important roles during the entire life cycle of higher plants including cell elongation, flowering and fruit set, seed germination, and many other developmental processes | Zea mays |