Cloned (Comment) | Organism |
---|---|
CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol | - |
Arabidopsis thaliana | |
(1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol | i.e. uniconazole | Arabidopsis thaliana | |
abscinazole-F1 | - |
Arabidopsis thaliana | |
methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana Columbia | - |
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana Columbia | - |
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana Columbia | - |
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Arabidopsis thaliana | the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview | ? | - |
? | |
additional information | Arabidopsis thaliana Columbia | the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93ZB2 | - |
- |
Arabidopsis thaliana Columbia | Q93ZB2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant CYP701A3 partially in microsomal membranes from Escherichia coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana Columbia | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana Columbia | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana Columbia | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview | Arabidopsis thaliana Columbia | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP701A3 | - |
Arabidopsis thaliana |
kaurene oxidase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid | Arabidopsis thaliana |
physiological function | kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid | Arabidopsis thaliana |