Cloned (Comment) | Organism |
---|---|
CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate | i.e. abscinazole-E1, orUT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, EC 1.14.13.93, but a weak inhibitor of CYP701A, both in vitro and in vivo | Oryza sativa | |
abscinazole-F1 | - |
Oryza sativa | |
DSI-505ME | - |
Oryza sativa | |
uniconazole | i.e. UNI | Oryza sativa | |
UT4 | - |
Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryza sativa | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa | CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin | ? | - |
? | |
additional information | Oryza sativa Nipponbare | CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Oryza sativa Nipponbare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant CYP701A partially from insect Sf9 cells in microsomes | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Oryza sativa | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Oryza sativa Nipponbare | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
additional information | CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin | Oryza sativa | ? | - |
? | |
additional information | CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin | Oryza sativa Nipponbare | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP701A | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
assay at | Oryza sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00097 | - |
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate | pH 7.25, 30°C, recombinant enzyme | Oryza sativa |