1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

This is an abbreviated version!
For detailed information about sarcosine oxidase (5,10-methylenetetrahydrofolate-forming), go to the full flat file.

Reaction

Synonyms

heterotetrameric sarcosine oxidase, HSO, sarcosine oxidase, sarcosine oxidase P, sarcosine: oxygen oxidoreductase (demethylating), sarcosine:oxygen oxidoreductase (demethylating), TSOX

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.3 With oxygen as acceptor

                1.5.3.24 sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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Results	in table
7	Cloned(Commentary)
32	Cofactor
3	Crystallization (Commentary)
1	General Information
1	General Stability
38	Inhibitors
4	kcat/KM [mM/s]
3	Ki Value [mM]
21	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
2	pH Optimum
1	pH Stability
16	Protein Variants
7	Purification (Commentary)
1	Reaction
18	Reference
6	Specific Activity [micromol/min/mg]
3	Storage Stability
34	Substrates and Products (Substrate)
15	Subunits
25	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.5.3.24 - sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

L-2-azetidinecarboxylic acid + O2

?

Corynebacterium sp. P-1

-

-

764122

-

-

?

L-pipecolic acid + O2

?

Corynebacterium sp. P-1

-

-

764122

-

-

?

L-proline + O2

?

Corynebacterium sp. P-1

-

poor substrate, the reduction of the enzyme with L-proline at pH 8.0 is not significant under aerobic conditions

764122

-

-

?

methylthioacetate + 5,6,7,8-tetrahydrofolate + O2

?

Corynebacterium sp. P-1

-

-

764129

-

-

?

N-ethyl-glycine + 5,6,7,8-tetrahydrofolate + O2

?

Corynebacterium sp. U-96

-

-

765024

-

-

?

N-methyl-L-alanine + 5,6,7,8-tetrahydrofolate + O2

?

Corynebacterium sp. U-96

-

-

765024

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + 2,6-dichlorophenolindophenol

?

Corynebacterium sp. U-96

-

-

765024

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + ferricyanide

?

Corynebacterium sp. U-96

-

-

765024

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

6 entries

sarcosine + 5,6,7,8-tetrahydrofolate + phenazine methosulfate

?

Corynebacterium sp. U-96

-

-

765024

-

-

?

sarcosine + 5-formyltetrahydrofolate + O2

?

Corynebacterium sp. P-1

-

-

764121

-

-

?

sarcosine + pteroyltetraglutamate + O2

?

Corynebacterium sp. P-1

-

-

764121

-

-

?

sarcosine + tetrahydropteroyltetraglutamate + O2

?

Corynebacterium sp. P-1

-

-

764121

-

-

?

additional information

?

-

4 entries

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Arthrobacter sp. 1-IN

-

-

764125

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Corynebacterium sp. P-1

-

-

764120, 764121, 764122, 764123, 764124, 764129

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Corynebacterium sp. U-96

-

-

765016, 765017, 765018, 765019, 765024, 765025, 765026, 765738

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Stenotrophomonas maltophilia

-

-

765237

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Stenotrophomonas maltophilia

Q3ZDQ8; Q3ZDR0; Q3ZDQ7; Q3ZDQ9

-

765715

-

-

?

sarcosine + 5,6,7,8-tetrahydrofolate + O2

glycine + 5,10-methylenetetrahydrofolate + H2O2

Stenotrophomonas maltophilia DSM 2701

Q3ZDQ8; Q3ZDR0; Q3ZDQ7; Q3ZDQ9

-

765715

-

-

?

additional information

?

-

Corynebacterium sp. P-1

-

no activity with D-proline

764122

-

-

-

additional information

?

-

Corynebacterium sp. P-1

-

no activity with NAD+ or NADP+ as electron acceptor in the presence or absence of various thiols (glutathione, coenzyme A, dithioerythritol)

764121

-

-

-

additional information

?

-

Corynebacterium sp. U-96

-

oxygen cannot be replaced by methylene blue or cytochrome c. The enzyme shows no activity with N,N-dimethylglycine, glycine, betaine, choline, 1,3-dimethylurea, 1-methylguanidine, 1-methylhydantoin, sarcosyl-glycine, and sarcosyl-sarcosine

765024

-

-

-

additional information

?

-

Corynebacterium sp. U-96

-

the reduction of the oxidized enzyme with dithiothreitol or its reoxidation with air shows slow kinetics. Moreover, the oxidized enzyme in an about 40% reduced state with dithiothreitol contains a flavin semiquinone comprising approximately 35% of the total flavin

765026

-

-

-