1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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For detailed information about sarcosine oxidase (5,10-methylenetetrahydrofolate-forming), go to the full flat file.

Reaction

Synonyms

heterotetrameric sarcosine oxidase, HSO, sarcosine oxidase, sarcosine oxidase P, sarcosine: oxygen oxidoreductase (demethylating), sarcosine:oxygen oxidoreductase (demethylating), TSOX

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.3 With oxygen as acceptor

1.5.3.24 sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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Results	in table
7	Cloned(Commentary)
32	Cofactor
3	Crystallization (Commentary)
1	General Information
1	General Stability
38	Inhibitors
4	kcat/KM [mM/s]
3	Ki Value [mM]
21	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
2	pH Optimum
1	pH Stability
16	Protein Variants
7	Purification (Commentary)
1	Reaction
18	Reference
6	Specific Activity [micromol/min/mg]
3	Storage Stability
34	Substrates and Products (Substrate)
15	Subunits
25	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.5.3.24 - sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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enzyme complexed with methylthioacetate, pyrrole 2-carboxylate and sulfite or sarcosine-reduced enzyme, using 0.1 M HEPES (pH 7.5) and 1.7 M ammonium sulfate

Corynebacterium sp. U-96

765018

hanging drop vapor diffusion method, using 17-20% (w/v) PEG 20 K, 100 mM Tris, pH 8.5, 40 mM sodium furoate and 10 mM Na2SO3

Stenotrophomonas maltophilia

Q3ZDQ8; Q3ZDR0; Q3ZDQ7; Q3ZDQ9

765715

native protein and the selenomethionine substituted protein, hanging drop vapor diffusion method, using about 17% (w/v) PEG 20000 with 100 mM Tris buffer (pH 8.5)

Stenotrophomonas maltophilia

765237