1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)
This is an abbreviated version!
For detailed information about sarcosine oxidase (5,10-methylenetetrahydrofolate-forming), go to the full flat file.
Reaction
sarcosine +
5,6,7,8-tetrahydrofolate +
O2=
glycine +
5,10-methylenetetrahydrofolate +
H2O2
Synonyms
heterotetrameric sarcosine oxidase, HSO, sarcosine oxidase, sarcosine oxidase P, sarcosine: oxygen oxidoreductase (demethylating), sarcosine:oxygen oxidoreductase (demethylating), TSOX
ECTree
KM Value
KM Value on EC 1.5.3.24 - sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)
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0.31
2,6-dichlorophenolindophenol
-
at pH 7.7 and 37°C
2.5
ferricyanide
-
at pH 7.7 and 37°C
11.4
N-ethyl-glycine
-
at pH 7.0 and 37°C
8.7
N-Methyl-L-alanine
-
at pH 8.3 and 37°C
1.67
phenazine methosulfate
-
at pH 7.7 and 37°C
0.0312
O2
-
mutant enzyme K171R, at pH 8.0 and 25°C
0.0329
O2
-
wild type enzyme, at pH 8.0 and 25°C
0.13
O2
-
at pH 7.7 and 37°C
0.386
O2
-
mutant enzyme K171A, at pH 8.0 and 25°C
0.73
O2
-
mutant enzyme K171D, at pH 8.0 and 25°C
1.4
sarcosine
-
wild type enzyme, at pH 8.0 and 25°C
1.47
sarcosine
-
mutant enzyme K171A, at pH 8.0 and 25°C
1.72
sarcosine
-
mutant enzyme K171R, at pH 8.0 and 25°C
2.75
sarcosine
-
mutant enzyme K171D, at pH 8.0 and 25°C
2.76
sarcosine
-
wild type enzyme, at pH 8.0 and 25°C
3.4
sarcosine
-
at pH 8.3 and 37°C
3.8
sarcosine
-
at pH 7.0 and 37°C
11.3
sarcosine
-
mutant enzyme Y269A, at pH 8.0 and 25°C
17.4
sarcosine
-
mutant enzyme K358R, at pH 8.0 and 25°C
72.6
sarcosine
-
mutant enzyme Y271F, at pH 8.0 and 25°C
1730
sarcosine
-
mutant enzyme Y271A, at pH 8.0 and 25°C
1.5.3.24 sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)
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