1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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For detailed information about sarcosine oxidase (5,10-methylenetetrahydrofolate-forming), go to the full flat file.

Reaction

Synonyms

heterotetrameric sarcosine oxidase, HSO, sarcosine oxidase, sarcosine oxidase P, sarcosine: oxygen oxidoreductase (demethylating), sarcosine:oxygen oxidoreductase (demethylating), TSOX

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.3 With oxygen as acceptor

1.5.3.24 sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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Results	in table
7	Cloned(Commentary)
32	Cofactor
3	Crystallization (Commentary)
1	General Information
1	General Stability
38	Inhibitors
4	kcat/KM [mM/s]
3	Ki Value [mM]
21	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
2	pH Optimum
1	pH Stability
16	Protein Variants
7	Purification (Commentary)
1	Reaction
18	Reference
6	Specific Activity [micromol/min/mg]
3	Storage Stability
34	Substrates and Products (Substrate)
15	Subunits
25	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 1.5.3.24 - sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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0.33

Corynebacterium sp. P-1

cell lysate, at pH 8.3 and 37°C

764120

0.38

Corynebacterium sp. U-96

crude enzyme, at pH 8.3 and 37°C

765024

12.2

Corynebacterium sp. P-1

after 37.1fold purification, at pH 8.3 and 37°C

764120

8.4

Corynebacterium sp. P-1

mutant enzyme H175A, pH and temperature not specified in the publication

764124

8.6

Corynebacterium sp. P-1

wild type enzyme, pH and temperature not specified in the publication

764124

8.93

Corynebacterium sp. U-96

after 23.5fold purification, at pH 8.3 and 37°C

765024