1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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For detailed information about sarcosine oxidase (5,10-methylenetetrahydrofolate-forming), go to the full flat file.

Reaction

Synonyms

heterotetrameric sarcosine oxidase, HSO, sarcosine oxidase, sarcosine oxidase P, sarcosine: oxygen oxidoreductase (demethylating), sarcosine:oxygen oxidoreductase (demethylating), TSOX

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.3 With oxygen as acceptor

1.5.3.24 sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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7	Cloned(Commentary)
32	Cofactor
3	Crystallization (Commentary)
1	General Information
1	General Stability
38	Inhibitors
4	kcat/KM [mM/s]
3	Ki Value [mM]
21	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
2	pH Optimum
1	pH Stability
16	Protein Variants
7	Purification (Commentary)
1	Reaction
18	Reference
6	Specific Activity [micromol/min/mg]
3	Storage Stability
34	Substrates and Products (Substrate)
15	Subunits
25	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 1.5.3.24 - sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)

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Corynebacterium sp. P-1

764124

ammonium sulfate precipitation, DE-52 column chromatography, phenyl Sepharose column chromatography, and Q-Sepharose column chromatography

Arthrobacter sp. 1-IN

764125

ammonium sulfate precipitation, Ultrogel AC A 34 Sepharose column chromatography, phenyl-Sepharose column chromatography, and DEAE-Sephacel column chromatography

Corynebacterium sp. P-1

764123

ammonium sulfate precipitation, Ultrogel ACA 34 chromatography, DEAE-cellulose column chromatography, and Phenyl-Sepharose column chromatography

Stenotrophomonas maltophilia

Q3ZDQ8; Q3ZDR0; Q3ZDQ7; Q3ZDQ9

765715

ammonium sulfate precipitation, Ultrogel AcA gel filtration, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and DEAE Sephacel column chromatography

Corynebacterium sp. P-1

764120

DEAE-cellulose colum chromatography, QAE-Sephadex A-50 gel filtration, DEAE Sephadex A-50 gel filtration, Ultrogel AcA34 gel filtration, and creatine-AH-Sepharose 4B colum chromatography

Corynebacterium sp. U-96

765024

phenyl-Sepharose column chromatography and Talon affinity resin column chromatography

Corynebacterium sp. P-1

764129