2.3.1.298: ultra-long-chain ceramide synthase

This is an abbreviated version!
For detailed information about ultra-long-chain ceramide synthase, go to the full flat file.

Reaction

Synonyms

(dihydro)ceramide synthase, ceramide synthase, ceramide synthase 3, CerS3, LASS3, LASS3-long, longevity assurance homologue 3, mammalian ceramide synthase 3, phytoceramide synthase, sphingoid base N-ultra-long-chain fatty acyl-coA transferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.298 ultra-long-chain ceramide synthase

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Results	in table
2	Activating Compound
4	AA Sequence
9	Cloned(Commentary)
5	Expression
14	General Information
6	Inhibitors
1	Localization
1	Metals/Ions
24	Natural Substrates/ Products (Substrates)
20	Organism
3	Pathway
3	pH Optimum
4	pI Value
1	Posttranslational Modification
2	Protein Variants
1	Reaction
19	Reference
35	Source Tissue
66	Substrates and Products (Substrate)
6	Subunits
37	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 2.3.1.298 - ultra-long-chain ceramide synthase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

11 entries

an ultra-long-chain fatty acyl-CoA + phytosphingosine

phytoceramide + CoA

Saccharomyces cerevisiae

-

-

753522

-

-

?

an ultra-long-chain fatty acyl-CoA + sphingosine

an ultra-long-chain ceramide + CoA

Mus musculus

-

the enzyme uses acyl-CoAs longer than 26 carbon atoms in length

753562

-

-

?

arachidoyl-CoA + dihydrosphingosine

CoA + N-arachidoyl-dihydrosphingosine

Mus musculus

Q1A3B0

weak activity

752701

-

-

?

arachidoyl-CoA + sphingosine

arachidoyl ceramide + CoA

Homo sapiens

-

-

754143

-

-

?

behenoyl-CoA + a sphingoid base

behenoyl ceramide + CoA

2 entries

behenoyl-CoA + dihydrosphingosine

CoA + N-behenoyldihydrosphingosine

2 entries

behenoyl-CoA + sphingosine

CoA + N-behenoylsphingosine

Homo sapiens

-

-

754143

-

-

?

cerotoyl-CoA + dihydrosphingosine

CoA + N-cerotoyl-dihydrosphingosine

Mus musculus

Q1A3B0

weak activity

752701

-

-

?

cerotoyl-CoA + sphingosine

cerotoyl ceramide + CoA

2 entries

dotriacontanoyl-CoA + sphinganine

dotriacontanoyl-ceramide + CoA

Mus musculus

-

-

752798

-

-

?

fatty acyl-CoA + dihydrosphingosine

ceramide + CoA

Mus musculus

Q1A3B0

-

752701

-

-

?

hexacosanoyl-CoA + a sphingoid base

hexacosanoyl ceramide + CoA

2 entries

hexacosanoyl-CoA + sphinganine

hexacosanoyl ceramide + CoA

2 entries

lignoceroyl-CoA + dihydrosphingosine

CoA + N-lignoceroyldihydrosphingosine

2 entries

lignoceroyl-CoA + sphingosine

lignoceroyl ceramide + CoA

2 entries

montanoyl-CoA + sphingosine

montanoyl ceramide + CoA

2 entries

palmitoyl-CoA + dihydrosphingosine

CoA + N-palmitoyldihydrosphingosine

Mus musculus

Q1A3B0

high activity

752701

-

-

?

palmitoyl-CoA + sphingosine

CoA + N-palmitoylsphingosine

2 entries

sphingosine + 2-hydroxybehenoyl-CoA

N-2-hydroxybehenoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + 2-hydroxyeicosanoyl-CoA

N-2-hydroxyeicosanoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + 2-hydroxylignoceroyl-CoA

N-2-hydroxylignoceroylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + 2-hydroxypalmitoyl-CoA

N-2-hydroxypalmitoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + 2-hydroxystearoyl-CoA

N-2-hydroxystearoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + behenoyl-CoA

N-behenoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + cerotoyl-CoA

N-cerotoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754626

-

-

?

sphingosine + eicosanoyl-CoA

N-eicosanoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + lignoceroyl-CoA

N-lignoceroylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + montanoyl-CoA

N-montanoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754626

-

-

?

sphingosine + palmitoyl-CoA

N-palmitoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

sphingosine + stearoyl-CoA

N-stearoylsphingosine + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

stearoyl-CoA + dihydrosphingosine

CoA + N-stearoyldihydrosphingosine

2 entries

stearoyl-CoA + sphingosine

CoA + N-stearoylsphingosine

2 entries

additional information

?

-

10 entries

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Danio rerio

A0A140LFW4

the enzyme exhibits a broad acyl chain preference synthesizing C26-C32 acyl chain ceramides

752924

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Gallus gallus

F1N8P2

the enzyme exhibits a broad acyl chain preference synthesizing C26-C32 acyl chain ceramides

752924

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Homo sapiens

-

-

754143, 754361

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Homo sapiens

Q8IU89

-

754361

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Homo sapiens

-

-

755196

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Homo sapiens

-

C26-C36

753770

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Homo sapiens

Q8IU89

the enzyme exhibits a broad acyl chain preference synthesizing C26-C32 acyl chain ceramides

752924

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Mus musculus

-

-

753771, 754160, 755196

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Mus musculus

-

C26-C34

752798

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Mus musculus

-

C26-C36

753770

-

-

?

an ultra-long-chain fatty acyl-CoA + a sphingoid base

an ultra-long-chain ceramide + CoA

Mus musculus

Q1A3B0

the enzyme exhibits a broad acyl chain preference synthesizing C26-C32 acyl chain ceramides

752924

-

-

?

behenoyl-CoA + a sphingoid base

behenoyl ceramide + CoA

Homo sapiens

-

-

755196

-

-

?

behenoyl-CoA + a sphingoid base

behenoyl ceramide + CoA

Mus musculus

-

-

755196

-

-

?

behenoyl-CoA + dihydrosphingosine

CoA + N-behenoyldihydrosphingosine

Homo sapiens

Q8IU89

-

754361

-

-

?

behenoyl-CoA + dihydrosphingosine

CoA + N-behenoyldihydrosphingosine

Mus musculus

Q1A3B0

-

752701

-

-

?

cerotoyl-CoA + sphingosine

cerotoyl ceramide + CoA

Homo sapiens

-

-

753770, 754143

-

-

?

cerotoyl-CoA + sphingosine

cerotoyl ceramide + CoA

Mus musculus

-

-

753770

-

-

?

hexacosanoyl-CoA + a sphingoid base

hexacosanoyl ceramide + CoA

Homo sapiens

-

-

755196

-

-

?

hexacosanoyl-CoA + a sphingoid base

hexacosanoyl ceramide + CoA

Mus musculus

-

-

755196

-

-

?

hexacosanoyl-CoA + sphinganine

hexacosanoyl ceramide + CoA

Mus musculus

-

-

752798

-

-

?

hexacosanoyl-CoA + sphinganine

hexacosanoyl ceramide + CoA

Mus musculus

-

main substrate

754160

-

-

?

lignoceroyl-CoA + dihydrosphingosine

CoA + N-lignoceroyldihydrosphingosine

Homo sapiens

Q8IU89

highest activity

754361

-

-

?

lignoceroyl-CoA + dihydrosphingosine

CoA + N-lignoceroyldihydrosphingosine

Mus musculus

Q1A3B0

-

752701

-

-

?

lignoceroyl-CoA + sphingosine

lignoceroyl ceramide + CoA

Homo sapiens

-

-

753770

-

-

?

lignoceroyl-CoA + sphingosine

lignoceroyl ceramide + CoA

Mus musculus

-

-

753770

-

-

?

montanoyl-CoA + sphingosine

montanoyl ceramide + CoA

Homo sapiens

-

bona fide substrate

753770

-

-

?

montanoyl-CoA + sphingosine

montanoyl ceramide + CoA

Mus musculus

-

bona fide substrate

753770

-

-

?

palmitoyl-CoA + sphingosine

CoA + N-palmitoylsphingosine

Homo sapiens

-

-

753770

-

-

?

palmitoyl-CoA + sphingosine

CoA + N-palmitoylsphingosine

Mus musculus

-

-

753770

-

-

?

stearoyl-CoA + dihydrosphingosine

CoA + N-stearoyldihydrosphingosine

Homo sapiens

Q8IU89

-

754361

-

-

?

stearoyl-CoA + dihydrosphingosine

CoA + N-stearoyldihydrosphingosine

Mus musculus

Q1A3B0

highest activity

752701

-

-

?

stearoyl-CoA + sphingosine

CoA + N-stearoylsphingosine

Homo sapiens

-

-

753770, 754143

-

-

?

stearoyl-CoA + sphingosine

CoA + N-stearoylsphingosine

Mus musculus

-

-

753770

-

-

?

additional information

?

-

Homo sapiens

Q8IU89

CERS3 transfers much longer chain fatty acids (C26, C28), forming highly hydrophobic ceramides that are a very important part of the water barrier function of skin

754626

-

-

?

additional information

?

-

Homo sapiens

Q8IU89

CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview

754626

-

-

?

additional information

?

-

Homo sapiens

-

isozyme CerS3 exhibits broad substrate specificity toward medium- to long-chain fatty acyl-CoAs (short-chain having less than C18, medium-chain with C18-C22, and long-chain with more than C22). In HEK-293T cells overproducing both CerS3 and 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase (FA2H), no 2-hydroxy-C16:0-ceramide is detected, but both medium- and long-chain 2-hydroxy-ceramides. CerS3 may use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates

754361

-

-

?

additional information

?

-

Homo sapiens

Q8IU89

isozyme CerS3 exhibits broad substrate specificity toward medium- to long-chain fatty acyl-CoAs (short-chain having less than C18, medium-chain with C18-C22, and long-chain with more than C22). In HEK-293T cells overproducing both CerS3 and 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase (FA2H), no 2-hydroxy-C16:0-ceramide is detected, but both medium- and long-chain 2-hydroxy-ceramides. CerS3 may use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates

754361

-

-

?

additional information

?

-

Homo sapiens

-

the enzyme also uses long-chain and very-long-chain fatty acyl-CoAs as substrate

753770

-

-

?

additional information

?

-

Homo sapiens

-

the enzyme can also use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates

754361

-

-

?

additional information

?

-

Homo sapiens

Q8IU89

the enzyme can also use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates

754361

-

-

?

additional information

?

-

Mus musculus

-

the enzyme also accepts long or very long fatty acyl-CoAs as substrate

753771

-

-

?

additional information

?

-

Mus musculus

-

the enzyme also uses long-chain and very-long-chain fatty acyl-CoAs as substrate

753770

-

-

?

additional information

?

-

Mus musculus

Q1A3B0

the enzyme exhibits relatively low substrate specificity toward fatty-acyl CoAs

752701

-

-

?