1.14.99.66: [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase

This is an abbreviated version!
For detailed information about [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase, go to the full flat file.

Reaction

Synonyms

amine oxidase flavin-containing domain 1, amine-oxidase flavin-containing domain 1, AOF1, AOF2, BHC110, BHC110/LSD1, CG9088, demethylase LSD1, dJARID1/Lid, H3K4 demethylase, H3K4me2 demethylase, H3K4me2/1 histone demethylase, histone demethylase, histone H3 K4 demethylase, histone H3 lysine 4 demethylase, histone H3K4 demethylase, histone lysine-specific demethylase 1, Jarid1, KDM1, KDM1A, KDM1B, KIAA0601, LD40310, Lid, LSD1, LSD1 demethylase, LSD1/KDM1, LSD1/KDM1A, LSD2, lysine demethylase 1B, lysine specific demethylase 1, lysine-specific demethylase 1, lysine-specific demethylase 1A, lysine-specific demethylase 2, lysine-specific demethylase-1, lysine-specific histone demethylase, lysine-specific histone demethylase 1, lysine-specific histone demethylase 1A, lysine-specific histone demethylase 1B, lysinespecific demethylase 1, More, SPR-5

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.99 Miscellaneous

                1.14.99.66 [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
6	Activating Compound
9	AA Sequence
3	Application
20	Cloned(Commentary)
32	Cofactor
11	Crystallization (Commentary)
292	Disease/ Diagnostics
2	Expression
86	General Information
9	IC50 Value
139	Inhibitors
7	kcat/KM [mM/s]
26	Ki Value [mM]
23	KM Value [mM]
47	Localization
3	Metals/Ions
3	Molecular Weight [Da]
97	Natural Substrates/ Products (Substrates)
123	Organism
20	PDB ID
9	pH Optimum
1	pH Range
1	Posttranslational Modification
21	Protein Variants
10	Purification (Commentary)
3	Reaction
64	Reference
86	Source Tissue
155	Substrates and Products (Substrate)
5	Subunits
161	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
18	Turnover Number [1/s]

Natural Substrates Products

print visible entries

print all entries

Natural Substrates Products on EC 1.14.99.66 - [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

a [histone H3]-N6,N6-dimethyl-L-lysine4 + O2 + 2 H2O

a [histone H3]-L-lysine4 + 2 formaldehyde + 2 H2O2

Homo sapiens

O60341

-

753561

-

-

?

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

5 entries

histone H3 N6-methyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 L-lysine4 + succinate + formaldehyde + CO2

3 entries

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

9 entries

[histone H3]-N6,N6-dimethyl-L-lysine 4 + acceptor + H2O

[histone H3]-N6-methyl-L-lysine 4 + formaldehyde + reduced acceptor

Homo sapiens

O60341

-

752795

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

753142

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

10 entries

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 acceptor + 2 H2O

[histone H3]-L-lysine4 + 2 formaldehyde + 2 reduced acceptor

Drosophila melanogaster

Q9VMJ7

overall reaction

754921

-

-

?

[histone H3]-N6,N6-methyl-L-lysine 4 + acceptor + H2O

[histone H3]-L-lysine 4 + formaldehyde + reduced acceptor

Homo sapiens

O60341

-

752795

-

-

?

[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

3 entries

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

8 entries

[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2

[histone H3]-L-lysine 9 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

753142

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

5 entries

additional information

?

-

14 entries

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

-

-

711769, 712706, 724316, 725593

-

-

?

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

745752

-

-

?

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

Mus musculus

-

-

725541, 725961

-

-

?

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

Mus musculus

Q6ZQ88

-

745804

-

-

?

histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2

Mus musculus C57/BL6J

-

-

725541

-

-

?

histone H3 N6-methyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

-

-

711769, 712706

-

-

?

histone H3 N6-methyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

745752

-

-

?

histone H3 N6-methyl-L-lysine4 + 2-oxoglutarate + O2

histone H3 L-lysine4 + succinate + formaldehyde + CO2

Mus musculus

Q6ZQ88

-

745804

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Caenorhabditis elegans

Q9XWP6

-

753349

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Drosophila melanogaster

Q9VMJ7

-

753349

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

744347, 744494, 745752, 752640, 752646, 752749, 752751, 753296, 753388

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

-

-

753388

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

753965, 754723, 754857, 755188, 755238

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus

Q6ZQ88

-

745771, 753349, 753388

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus

Q8CIG3

-

752868

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus C57BL/6

Q6ZQ88

-

753388

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus C57BL/6J

Q6ZQ88

-

753349

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Arabidopsis thaliana

-

-

697909

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Arabidopsis thaliana

Q8VXV7

-

696261

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Caenorhabditis elegans

-

-

697241, 697909

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Drosophila melanogaster

-

-

697909

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Drosophila melanogaster

-

dimethyl-H3K4 is an activation markers for gene expression

701350

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Homo sapiens

-

-

697909, 700154

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Homo sapiens

O75164

dimethyl-H3K4 is an activation markers for gene expression

701350

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Homo sapiens

-

lack of H3K4diMe is possibly due to complex epigenetic regulation involving Ash2 and LSD1

698198

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Mus musculus

-

-

697355, 700158, 700991

-

-

?

[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2

[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2

Mus musculus

-

demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis

700397

-

-

?

[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Drosophila melanogaster

-

-

701350

-

-

?

[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

O75164

-

701350

-

-

?

[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Mus musculus

-

demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis

700397

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Caenorhabditis elegans

Q9XWP6

-

753349

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Drosophila melanogaster

Q9VMJ7

-

753349

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

744347, 744494, 745752, 752640, 752646, 752749, 752751, 753296, 753388

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

-

-

753388

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Homo sapiens

O60341

-

753965, 754723, 754857, 755188, 755238

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus

Q6ZQ88

-

745771, 753349, 753388

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus

Q8CIG3

-

752868

-

-

?

[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2

[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2

Mus musculus C57BL/6J

Q6ZQ88

-

753349

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Arabidopsis thaliana

-

-

697909

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Arabidopsis thaliana

Q8VXV7

-

696261

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Caenorhabditis elegans

-

-

697909

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Drosophila melanogaster

-

-

697909

-

-

?

[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2

[histone H3]-L-lysine4 + succinate + formaldehyde + CO2

Homo sapiens

-

-

697909

-

-

?

additional information

?

-

Drosophila melanogaster

-

LSD1 interacts with several interaction partners and transcription factors for performing its role in gene regulation, overview

701350

-

-

?

additional information

?

-

Homo sapiens

O60341

functional interplay between histone demethylase and histone deacetylase

754723

-

-

?

additional information

?

-

Homo sapiens

-

LSD1 forms a complex with CoREST and histone deacetylase 1. LSD1 mediates the transrepressive function of TLX, an orphan nuclear receptor, also called NR2E1, that regulates the expression of target genes by functioning as a constitutive transrepressor, through direct interaction via its SWIRM and amine oxidase domains. Physiological significance of TLX in the cytodifferentiation of neural cells in the brain

700154

-

-

?

additional information

?

-

Homo sapiens

-

LSD1 forms a stable complex with Rb, but with E2F1, cell cycle regulatory proteins. LSD1 binds to Epstein-Barr virus C promoter Cp in a cell cycle-dependent manner, as do the the cell cycle regulatory proteins E2F1 and Rb. Rb and LSD1 binding to Cp increase after the S phase, corresponding to a decrease in histone H3 K4 methylation and Cp transcription, overview

699898

-

-

?

additional information

?

-

Homo sapiens

-

LSD1 interacts with CoREST, a co-repressor protein that binds REST and recruits other histone-modifying enzymes such as histone deacetylases 1 ? 2. The function of the LSD1–CoREST–histone deacetylase subcomplex in transcriptional repression events is not limited to REST-regulated neuronal genes, but can be extended to other contexts such as hematopoietic differentiation and the telomerase reverse transcriptase genes

697909

-

-

?

additional information

?

-

Homo sapiens

O75164

LSD1 interacts with several interaction partners and transcription factors for performing its role in gene regulation, overview. LSD1 forms a complex with CoREST, structure with bound histone H3 peptide substrate, overview. LSD1 tightly associates with the CoREST C-terminal SANT domain. This intermolecular association is mediated by the LSD1 tower domain, whose alpha-helices are embraced by a helical segment of CoREST, generating an intermolecular helical coil. The histone H3 N-terminal peptide binds deeply in the LSD1 amine oxidase domain in proximity to the flavin cofactor

701350

-

-

?

additional information

?

-

Homo sapiens

-

LSD1 removes the methyl groups from lysines 4 and 9 of histone 3 with the generation of formaldehyde from the methyl group

724316

-

-

?

additional information

?

-

Homo sapiens

O60341

LSD1 directly binds to the promoter of P21 where it catalyzes H3K4me2 demethylation. FEZF1-AS1, a 2564 bp RNA overexpressed in gastric cancer, epigenetically represses the expression of P21 via binding with LSD1

745752

-

-

?

additional information

?

-

Homo sapiens

O60341

lysine-specific demethylase 1(LSD1) demethylates mono- and dimethylated residues of lysine-4 on histone H3 (H3K4me1 and H3K4me2) and lysine-9 on histone H3 (H3K9me1 and H3K9me2)

745752

-

-

?

additional information

?

-

Mus musculus

-

amine oxidase flavin-containing domain 1, AOF1, also called lysine demethylase 1B , KDM1B, is a protein related to the lysine demethylase KDM1or LSD1, it functions as a H3K4 demethylase and is required for de novo DNA methylation of some imprinted genes in oocytes

700397

-

-

?

additional information

?

-

Mus musculus

-

p53 directly interacts with LSD1 to alter chromatin structure and confer developmental repression of the tumor marker alpha-fetoprotein, AFP, p53 and LSD1 cooccupy a p53 response element, concomitant with dimethylated histone H3 lysine 4 demethylation and postnatal repression of AFP transcription, overview

700158

-

-

?

additional information

?

-

Mus musculus

-

the enzyme is organized in histone demethylase complexes containing LSD1, RE1 silencing transcription factor corepressor, CoREST, histone deacetylase 1, HDAC1, and histone deacetylase 2 in erythroleukemia and T cell leukemia cells. the Complex interacts with TAL, a critical transcription factor required for hematopoiesis, overview. The enzymatic domain of LSD1 plays an important role in repressing the TAL1-directed transcription, overview. TAL1-associated LSD1 and HDM activity are dynamically regulated during hematopoiesis

700991

-

-

?

additional information

?

-

Mus musculus

Q6ZQ88

LSD1 demethylates mono- and dimethylated H3K4 and H3K9, but does not alter trimethylated H3K4 and H3K9

745804

-

-

?

additional information

?

-

Mus musculus

-

LSD1 demethylates mono- and dimethylated H3K4 and H3K9, but does not alter trimethylated H3K4 and H3K9

745804

-

-

?