1.14.14.145: abieta-7,13-dien-18-ol hydroxylase

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For detailed information about abieta-7,13-dien-18-ol hydroxylase, go to the full flat file.

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Synonyms

abieta-7,13-dien-18-ol hydroxylase, abietadienol/abietadienal oxidase, CYP720B1, CyPA, CYPA1, CYPA2, CyPB, CYPC, CYPD, EC 1.14.13.109, PtAO

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.145 abieta-7,13-dien-18-ol hydroxylase

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Results	in table
1	Activating Compound
78	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
5	Cofactor
5	IC50 Value
6	Inhibitors
16	KM Value [mM]
5	Localization
8	Natural Substrates/ Products (Substrates)
12	Organism
3	Pathway
4	pH Optimum
2	pH Range
4	Reaction
11	Reference
5	Source Tissue
1	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
19	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

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Cloned on EC 1.14.14.145 - abieta-7,13-dien-18-ol hydroxylase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, de novo diterpenoid formation in metabolically engineered yeast, overview

Pinus taeda

Q50EK3, Q50EK4, Q50EK5, Q50EK6

676795

CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Pinus taeda

Q50EK3, Q50EK4, Q50EK5, Q50EK6

676795

CYPA, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Pinus taeda

Q50EK3, Q50EK4, Q50EK5, Q50EK6

676795

CYPA1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Picea sitchensis

Q50EK0, Q50EK1

676795

CYPA2, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Picea sitchensis

Q50EK0, Q50EK1

676795

CYPB, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Pinus taeda

Q50EK3, Q50EK4, Q50EK5, Q50EK6

676795

CYPC, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis

Pinus taeda

Q50EK3, Q50EK4, Q50EK5, Q50EK6

676795

expression of GFP-fusion protein in transiently transformed tobacco cells. The first 48 amino of PtAO are fused to the N-terminus of green fluorescent protein (GFP) and expressed in tobacco under control of Cauliflower Mosaic Virus 35S promoter

Pinus taeda

Q50EK6

666567