2.3.1.9: acetyl-CoA C-acetyltransferase

This is an abbreviated version!
For detailed information about acetyl-CoA C-acetyltransferase, go to the full flat file.

Reaction

2 acetyl-CoA =

Synonyms

2-methylacetoacetyl-CoA thiolase, 3-ketoacyl-CoA (T1)-like thiolase, 3-ketoacyl-CoA thiolase, 3-ketoacyl-coenzyme A thiolase, 3-oxothiolase, A1887, AACT, AACT1, AACT2, ACAT, ACAT1, ACAT2, ACCT, acetoacetyl CoA thiolase, acetoacetyl-CoA acetyltransferase, acetoacetyl-CoA C-acetyltransferase, acetoacetyl-CoA thiolase, acetoacetyl-CoA thiolase T2, acetyl coenzyme A thiolase, acetyl-CoA acetyltransferase, acetyl-CoA C-acetyltransferase, acetyl-CoA:N-acetyltransferase, acetyltransferase, acetyl coenzyme A, ACOAT, ACTRANS, AFUB_000550, AtoB, beta-acetoacetyl coenzyme A thiolase, beta-ketoacyl-CoA thiolase, beta-ketothiolase, CT, cytosolic acetoacetyl-CoA thiolase, cytosolic acetoacetyl-CoA thiolase 1, cytosolic acetoacetyl-CoA thiolase 2, ERG10, Erg10A, HFX_1022, HFX_1023, HFX_6003, HFX_6004, KACT, MmgA, Msed_0656, MSM-13 thiolase, OsAT1, phaA, ReH16_B0759, thiolase II, ThL, Tneu_0249, type II thiolase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.9 acetyl-CoA C-acetyltransferase

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Results	in table
4	Activating Compound
45649	AA Sequence
7	Application
1	CAS Registry Number
26	Cloned(Commentary)
1	Cofactor
15	Crystallization (Commentary)
275	Disease/ Diagnostics
3	Expression
32	General Information
6	General Stability
1	IC50 Value
65	Inhibitors
2	kcat/KM [mM/s]
19	Ki Value [mM]
87	KM Value [mM]
38	Localization
8	Metals/Ions
69	Molecular Weight [Da]
86	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
178	Organism
1	Oxidation Stability
50	Pathway
274	PDB ID
22	pH Optimum
4	pH Range
4	pI Value
55	Protein Variants
41	Purification (Commentary)
14	Reaction
6	Reaction Type
101	Reference
1	Renatured (Commentary)
74	Source Tissue
29	Specific Activity [micromol/min/mg]
12	Storage Stability
179	Substrates and Products (Substrate)
57	Subunits
128	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
8	Temperature Stability [°C]
28	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.3.1.9 - acetyl-CoA C-acetyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, less than 0.2 mg/ml protein, 50 mM Tris, pH 7.2, 0.5 mM dithiothreitol, 8 d, 60-70% loss of activity

Bos taurus

-

487675

-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM 1 mM, EDTA, 15 d, loss of more than 50% activity

Rhizobium sp.

-

487695

-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 15 d, loss of 20% activity

Rhizobium sp.

-

487695

-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 20% glycerol, 60 d, loss of less than 20% activity

Rhizobium sp.

-

487695

-20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol

Zoogloea ramigera

-

487668

-20°C, in dilute solution, less than 0.5 mg/ml protein, 24 h, complete loss of activity

Bradyrhizobium japonicum

-

487687

-90°C, 12 months

Gallus gallus

-

487669, 487671

0°C, 0.05 M Tris-HCl, pH 7.8, 2 mM dithiothreitol, 1 mM EDTA, 20% glycerol, 6 weeks, 50% loss of activity

Escherichia coli

-

487690

25°C, 1 week, 4.5 M KCl, no loss of activity

Halobacterium sp.

-

487703

25°C, 72 h, 200 mM KCl, 90% loss of activity

Halobacterium sp.

-

487703

4°C, 1 mM dithiothreitol, 10% v/v glycerol, N2 atmosphere, 1 month, less than 10% loss of activity

Clostridium acetobutylicum

-

487684