1.14.20.B3: cycloclavine dioxygenase (alternatively: multifunctional dioxygenase EasH)
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Reaction
Synonyms
EasH
ECTree
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General Information
General Information on EC 1.14.20.B3 - cycloclavine dioxygenase (alternatively: multifunctional dioxygenase EasH)
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metabolism
a mechanism of direct abstraction of hydrogen at C10 by the iron-oxo species followed by rearrangement is suggested
physiological function
EasH is responsible for a cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine
physiological function
enzymes EasA, EasG and EasH catalyze the formation of alkaloid cycloclavine from chanoclavine-I aldehyde in presence of FeII, NADP+, NADPH, ascorbic acid, and 2-oxoglutarate. The reaction of EasA and EasG with chanoclavine-I aldehyde in absence of EasH yields festuclavine
physiological function
non-heme iron, 2-oxoglutarate-dependent oxidase EasH works together with flavoenzyme EasA and NADPH-dependent oxidoreductase EasG to catalyze the conversion of chanoclavine-I aldehyde to cycloclavine. EasH is responsible for conversion of a 6-membered ring of the product of EasA to the fused 5-3 ring system observed in the cycloclavine molecule