1.14.19.67: salutaridine synthase

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For detailed information about salutaridine synthase, go to the full flat file.

Reaction

Synonyms

(R)-reticuline dehydrogenase, (R)-reticuline oxidase (C-C phenol-coupling), CYP719B1, dehydrogenase, (R)-reticuline, EC 1.1.3.35, EC 1.14.21.4, PsSAS, SalSyn, salutaridine synthase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

                1.14.19.67 salutaridine synthase

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Results	in table
1	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
10	Cofactor
6	Disease/ Diagnostics
1	General Information
3	Inhibitors
4	KM Value [mM]
11	Localization
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
11	Organism
6	Pathway
3	pH Optimum
1	pH Range
2	Purification (Commentary)
2	Reaction
5	Reaction Type
11	Reference
11	Source Tissue
4	Storage Stability
11	Substrates and Products (Substrate)
2	Subunits
11	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.19.67 - salutaridine synthase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

full-length CYP719B1 cDNA is generated by amplification out of bacterial vector pCR2.1 and inserted into baculovirus transfer vector pVL1392

Papaver somniferum

B1NF18

698930

salutaridine synthase (PsSAS), salutaridine reductase (PsSAR) and salutaridinol acetyltransferase (PsSAT) are functionally coexpressed in Saccharomyces cerevisiae and optimization of the pH conditions allowed for productive spontaneous rearrangement of salutaridinol-7-O-acetate and synthesis of thebaine from (R)-reticuline. A 7-gene pathway for the production of codeine and morphine from (R)-reticuline is reconstituted. Yeast cell feeding assays using (R)-reticuline, salutaridine or codeine as substrates show that all enzymes are functionally coexpressed in yeast and that activity of salutaridine reductase and codeine-O-demethylase likely limit flux to morphine synthesis. The results of this study describe a significant advance for the synthesis of morphinans in Saccharomyces cerevisiae and pave the way for their complete synthesis in recombinant microbes

Papaver somniferum

B1NF18

743574