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BRENDA support

1.14.14.178: steroid 22S-hydroxylase

This is an abbreviated version!
For detailed information about steroid 22S-hydroxylase, go to the full flat file.

Reaction

a C27-steroid
+
O2
 +
=
a (22S)-22-hydroxy-C27-steroid
 + 2 H2O +

Synonyms

AOD4, BR C-22 hydroxylase, C-22 hydroxylase, CYP724A1, CYP724B2, CYP90B1, CYP90B3, DWARF4, Dwf4, steroid 22-hydroxylase, steroid 22-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.178 steroid 22S-hydroxylase

Cloned

Cloned on EC 1.14.14.178 - steroid 22S-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis plants ectopically overexpressing DWF4 (AOD4) are generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes are characterized. The hypocotyl length of both light and dark-grown AOD4 seedlings is increased dramatically as compared to wild type. At maturity, inflorescence height increases >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced
expressed in Arabidopsis thaliana
expressed in Brassica napus
-
expressed in Escherichia coli JM109 cells
-
expression in Escherichia coli
-
expression in insect cell using a baculovirus expression system
gene DWF4, semi-quantitative reverse-transcriptase PCR expression analysis