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1.14.14.141: psoralen synthase

This is an abbreviated version!
For detailed information about psoralen synthase, go to the full flat file.

Word Map on EC 1.14.14.141

Reaction

(+)-marmesin
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
psoralen
 +
[oxidized NADPH-hemoprotein reductase]
 +
acetone
 + 2 H2O

Synonyms

CYP71AJ1, CYP71AJ2, CYP71AJ3, EC 1.14.13.102, psoralen synthase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.141 psoralen synthase

Cloned

Cloned on EC 1.14.14.141 - psoralen synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
-
expressed in yeast strain WAT 11
gene CYP71AJ1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the wild-type enzyme in Escherichia coli and Saccharomyces cerevisiae strain WAT11 requiring swapping the N-terminal membrane anchor domain with that of CYP73A1, expression of the enzyme mutant in yeast cells