2.4.1.252: GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
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For detailed information about GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase, go to the full flat file.
Reaction
Synonyms
AceA, alpha-mannosyltransferase AceA, GDP-mannosyltransferase, gumH
ECTree
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Cloned
Cloned on EC 2.4.1.252 - GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
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expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells
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overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein
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recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain
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