Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid
GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid
-
GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphophytanol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphophytanol
-
synthetic phytanyl-pyrophosphate-linked cellobiose functions as an acceptor for the AceA mannosyltransferase reaction in vitro. Neither the chain length nor the saturation state, notably of the first isoprene unit, are critical to the AceA mannosyltransferase activity
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
additional information
?
-
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
-
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity
characterisation of the in vitro reaction product
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-phsophate lipid carrier by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
-
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
the enzyme is involved in biosynthesis of xanthan
-
-
?
additional information
?
-
-
AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue
-
-
?
additional information
additional information
-
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
the enzyme is involved in biosynthesis of xanthan
-
-
?
additional information
additional information
-
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
-
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
the gum gene cluster constitutes an operon expressed from multiple promoters located upstream of gumB and gumG, respectively. The existence of two internal promoters is newly identified in the Xanthomonas oryzae gum gene cluster through the insertion mutations with the rrnB transcriptional terminator and RT-PCR analyses
malfunction
-
from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumH completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Oligosaccharides obtained from gumH- strain XcH can be resolved into two components: a compound with the same mobility as the disaccharide cellobiose and glucose. XcH cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose do not incorporate radioactivity into the 1203 extract
malfunction
significantly attenuated production of xanthan in knock-out mutant strain
physiological function
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan
physiological function
the enzyme is involved in acetan polysaccharide synthesis. AceA can complement a Xanthomonas campestris mutant that is deficient in the homologous gene gumH (involved in xanthan biosynthesis)
physiological function
-
the enzyme is involved in biosynthesis of xanthan
physiological function
-
the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
physiological function
-
GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Lellouch, A.C.; Watt, G.M.; Geremia, R.A.; Flitsch, S.L.
Phytanyl-pyrophosphate-linked substrate for a bacterial alpha-mannosyltransferase
Biochem. Biophys. Res. Commun.
272
290-292
2000
Komagataeibacter xylinus
brenda
Lee, C.K.; Lee, B.M.; Cho, J.Y.
Identification of new internal promoters of the Xanthomonas oryzae pathovar oryzae gum gene cluster
Biotechnol. Lett.
30
521-527
2008
Xanthomonas oryzae (Q5GXZ4)
brenda
Kim, S.Y.; Kim, J.G.; Lee, B.M.; Cho, J.Y.
Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae
Biotechnol. Lett.
31
265-270
2008
Xanthomonas oryzae (Q5GXZ4)
brenda
Ielpi, L.; Couso, R.O.; Dankert, M.A.
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris
J. Bacteriol.
175
2490-2500
1993
Xanthomonas campestris
brenda
Petroni, E.A.; Ielpi, L.
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase gene from Acetobacter xylinum
J. Bacteriol.
178
4814-4821
1996
Komagataeibacter xylinus (Q44571), Komagataeibacter xylinus
brenda
Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Phler, A.; Ielpi, L.
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence
J. Bacteriol.
180
1607-1617
1998
Xanthomonas campestris
brenda
Abdian, P.L.; Lellouch, A.C.; Gautier, C.; Ielpi, L.; Geremia, R.A.
Identification of essential amino acids in the bacterial
J. Biol. Chem.
275
40568-40575
2000
Komagataeibacter xylinus
brenda
Geremia, R.A.; Roux, M.; Ferreiro, D.U.; Dauphin-Dubois, R.; Lellouch, A.C.; Ielpi, L.
Expression and biochemical characterisation of recombinant AceA, a bacterial alpha-mannosyltransferase
Mol. Gen. Genet.
261
933-940
1990
Komagataeibacter xylinus
brenda
Muniz, J.R.; Alves, C.A.; de Pieri, C.; Beltramini, L.M.; Selistre-de-Araujo, H.S.; Vettore, A.L.; da Silva, F.R.; Arruda, P.; Garratt, R.C.; Oliva, G.; Souza, D.H.
Overexpression, purification, biochemical characterization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa
Biochem. Biophys. Res. Commun.
315
485-492
2004
Xylella fastidiosa
brenda
Alves, C.A.; Pedroso, M.M.; de Moraes, M.C.; Souza, D.H.; Cass, Q.B.; Faria, R.C.
Real-time investigation of mannosyltransferase function of a Xylella fastidiosa recombinant GumH protein using QCM-D
Biochem. Biophys. Res. Commun.
408
571-575
2011
Xylella fastidiosa
brenda