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2.3.1.23: 1-acylglycerophosphocholine O-acyltransferase

This is an abbreviated version!
For detailed information about 1-acylglycerophosphocholine O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.23

Reaction

acyl-CoA
+
1-acyl-sn-glycero-3-phosphocholine
=
CoA
 +
1,2-diacyl-sn-glycero-3-phosphocholine

Synonyms

1-acyl-sn-glycero-3-phosphocholine acyltransferase, 1-acylglycerol-3-phosphate O-acyltransferase 8, 1-acylglycerophosphocholine acyltransferase, 1-AGP acyltransferase 8, 1-AGPAT 8, 1-AGPAT7, acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase, acyl coenzyme A-monoacylphosphatidylcholine acyltransferase, acyl-CoA:1-acyl-glycero-3-phosphocholine transacylase, acyl-CoA:glycerophosphocholine acyltransferase, acyl-CoA:lysocardiolipin acyltransferase 1, acyl-CoA:lysophosphatidylcholine acyltransferase, acyl-CoA:lysophosphatidylethanolamine acyltransferase 2, acyl-CoA:lysophospholipid acyltransferase, acyltransferase, lysolecithin, AGPAT7, AGPAT8, Alcat1, ALE1, At1g78690, AtLPLAT2, AYTL1, AYTL2, AYTL3, CF3, CG18445, CsLPCAT, GPCAT, hPrdx6, Lclat1, LPAT, LPC acyltransferase, LPCAT, LPCAT1, LPCAT2, LPCAT3, LPCAT4, LPEAT2, LPG acyltransferase, LPGAT, LPGAT1, LPLAT, LPLAT5, lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1, LYCAT, lyso-PAF acetyltransferase, lyso-PC acyltransferase, lyso-PC acyltransferase 1, lyso-phosphatidylcholine acyltransferase 1, lysolecithin acyltransferase, lysoPC acyltransferase, lysophosphatide acyltransferase, lysophosphatidylcholine acyl transferase, lysophosphatidylcholine acyltransferase, lysophosphatidylcholine acyltransferase 1, lysophosphatidylcholine acyltransferase 3, lysophosphatidylcholine acyltransferase 4, lysophosphatidylethanolamine acyltransferase 2, lysophosphocholine acyltransferase 1, lysophosphocholine acyltransferase 2, lysophosphocholine acyltransferase 3, lysophosphocholine acyltransferase 4, lysophospholipid acyltransferase, lysophospholipid:acyl-CoA acyltransferase, mboa-6, MBOAT protein Nes, MBOAT5, membrane-bound O-acyltransferase 5, mLPEAT2, More, mPrdx6, N-acylphosphatidylethanolamine synthase, NAPE synthase, PCAT1, PCAT2, PGAT, rPrdx6, YALI0E16797g, yeast tafazzin, Ypr140wp

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.23 1-acylglycerophosphocholine O-acyltransferase

Purification

Purification on EC 2.3.1.23 - 1-acylglycerophosphocholine O-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cibacron blue agarose, DEAE cellulose, Matrex green A, 1-acylglycerophosphoinositol O-acyltransferase
-
copurification of His-tagged or GST-tagged enzyme with His-tagged StarD10 protein from Escherichia coli by affinity chromatography
gel filtration in the presence of 7.25 M urea, trypsin treatment
-
partial purification with palmitoyl-CoA-agarose affinity chromatography
-
partially purified
partially purified from vesicles
-
reactive blue 2-agarose, DEAE-Cellulose, Matrex green A
-
recombinant enzyme hPrdx6 by anion exchange chromatography
recombinant enzyme rPrdx6 by anion exchange chromatography
recombinant Twin-Strep-tagged enzyme from Sf9 insect cells by membrane preparation, affinity chromatography, and gel filtration
solubilization and selective extraction with lysolecithin analogs at low concentration
-
solubilization with CHAPS
-
solubilized by detergent effect of substrates lysolecithin and oleoyl-CoA, ratio 1:1
-
solubilized with CHAPS
-
solubilized with lysolecithin, reactive Blue-2-agarose, DEAE cellulose, Matrex Green A
-
solubilized with Triton X-100
-
Spharose 6B, Mono Q, severe loss of activity is partially avoided by incorporation of 20% glycerol in the elution buffer
-
Ultrogel AcA 34
-