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1.5.3.26: fructosyl amine oxidase (fructosamine-forming)

This is an abbreviated version!
For detailed information about fructosyl amine oxidase (fructosamine-forming), go to the full flat file.

Reaction

an N-(1-deoxy-D-fructos-1-yl)amine
+
O2
+
H2O
=
(1-deoxy-D-fructos-1-yl)amine
+
an aldehyde
+
H2O2

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.26 fructosyl amine oxidase (fructosamine-forming)

Purification

Purification on EC 1.5.3.26 - fructosyl amine oxidase (fructosamine-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme can be solubilized in active form using 10 mM 3-[(3-chlolamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate aided by 2 M NaCl and 10% glycerol (27% optimal solubilization yield)
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