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1.3.1.91: tRNA-dihydrouridine20 synthase [NAD(P)+]

This is an abbreviated version!
For detailed information about tRNA-dihydrouridine20 synthase [NAD(P)+], go to the full flat file.

Word Map on EC 1.3.1.91

Reaction

5,6-dihydrouracil20 in tRNA
+
NAD(P)+
=
uracil20 in tRNA
 +
NAD(P)H
 +
H+

Synonyms

dihydrouridine synthase, dihydrouridine synthase 2, dihydrouridine synthase C, Dus, DUS 2, DUS2, Dus2p, DusA, DusB, DusC, EcoDusC, hDUS2, HsDus2, SMM1, tRNA-dihydrouridine synthase 2, tRNA-dihydrouridine synthases

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.91 tRNA-dihydrouridine20 synthase [NAD(P)+]

Cloned

Cloned on EC 1.3.1.91 - tRNA-dihydrouridine20 synthase [NAD(P)+]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21DE3 Star cells
expression in Escherichia coli
expression in Escherichia coli. There is a higher proportion of tRNAGly in Escherichia coli expressinf DUS2
recombinant expression of His-tagged enzyme as wild-type and selenomethionine-labeled proteins in Escherichia coli strains BL21(DE3) and B834 (DE3), respectively
recombinant expression of His-tagged enzyme, functional complementation of an enzyme-deficient Saccharomyces cerevisiae mutant by expression of enzyme domains HsDus2dusD and HsDus2dsRBD, both domains are required for activity
recombinant expression of wild-type and selenomethionine-labeled hDus2 1-340 fragment, comprising the hDus2 catalytic and tRNA-recognition domains, in Escherichia coli strains BL21(DE3) and B834(DE3), respectively