Literature summary for 1.3.1.91 extracted from

Bou-Nader, C.; Montemont, H.; Guerineau, V.; Jean-Jean, O.; Bregeon, D.; Hamdane, D.
Unveiling structural and functional divergences of bacterial tRNA dihydrouridine synthases Perspectives on the evolution scenario (2018), Nucleic Acids Res., 46, 1386-1394 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 1.6 M lithium sulfate and 100 mM HEPES pH 7.5	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	2 mM used in assay conditions	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uracil20 in tRNA + NADH + H+	Escherichia coli	-	5,6-dihydrouracil20 in tRNA + NAD+	-	?
uracil20a in tRNA + NADH + H+	Escherichia coli	-	5,6-dihydrouracil20a in tRNA + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P32695	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uracil20 in tRNA + NADH + H+	-	Escherichia coli	5,6-dihydrouracil20 in tRNA + NAD+	-	?
uracil20a in tRNA + NADH + H+	-	Escherichia coli	5,6-dihydrouracil20a in tRNA + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
DusA	isoform	Escherichia coli
tRNA-dihydrouridine synthases	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Escherichia coli
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)