1.14.14.99: (S)-limonene 3-monooxygenase

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For detailed information about (S)-limonene 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

(-)-limonene 3-hydroxylase, (-)-limonene 3-monooxygenase, (-)-limonene,NADPH:oxygen oxidoreductase (3-hydroxylating), (-)-limonene-3-hydroxylase, EC 1.14.13.47, L3oh, limonene-3-hydroxylase, More, oxygenase, (-)-limonene 3-mono-, PM17

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.99 (S)-limonene 3-monooxygenase

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Results	in table
3	Activating Compound
6	AA Sequence
1	Application
1	CAS Registry Number
5	Cloned(Commentary)
6	Cofactor
1	Expression
3	General Stability
9	Inhibitors
1	KM Value [mM]
4	Localization
4	Natural Substrates/ Products (Substrates)
13	Organism
3	Pathway
3	pH Optimum
2	pH Range
1	Protein Variants
2	Purification (Commentary)
2	Reaction
3	Reaction Type
13	Reference
5	Source Tissue
2	Specific Activity [micromol/min/mg]
25	Substrates and Products (Substrate)
12	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.14.14.99 - (S)-limonene 3-monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Mentha x piperita

-

390087

expression in Escherichia coli

Mentha x gracilis

-

660093

expression in Escherichia coli and Saccharomyces cerevisiae

Mentha x piperita

-

390088

expression of the chimeric protein in Escherichia coli

Perilla frutescens

-

713236

overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition

Mentha x piperita

Q9XHE7

745019