1.14.14.163: (S)-1-hydroxy-N-methylcanadine 13-hydroxylase
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Reaction
Synonyms
1-hydroxy-N-methylcanadine 13-hydroxylase, CYP82X2
ECTree
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Cloned
Cloned on EC 1.14.14.163 - (S)-1-hydroxy-N-methylcanadine 13-hydroxylase
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expression in Saccharomyces cerevisiae. Owing to difficulties with heterologous expression in yeast, CYP82X2 is synthesized with the first 60 N-terminal amino acids replaced with the first 43 N-terminal amino acids from the lettuce cytochrome P450 germacrene A oxidase
expression in Saccharomyces cerevisiae. Reconstitution of the noscapine gene cluster in Saccharomyces cerevisiae to achieve the microbial production of noscapine and related pathway intermediates