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1.14.14.110: 2-hydroxy-1,4-benzoxazin-3-one monooxygenase

This is an abbreviated version!
For detailed information about 2-hydroxy-1,4-benzoxazin-3-one monooxygenase, go to the full flat file.

Reaction

3-hydroxyindolin-2-one
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

benzoxazinone N-monooxygenase, BX5, Cyp71C3, EC 1.14.13.140

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.110 2-hydroxy-1,4-benzoxazin-3-one monooxygenase

General Stability

General Stability on EC 1.14.14.110 - 2-hydroxy-1,4-benzoxazin-3-one monooxygenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity
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