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1.14.14.108: 2,5-diketocamphane 1,2-monooxygenase

This is an abbreviated version!
For detailed information about 2,5-diketocamphane 1,2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.108

Reaction

(+)-bornane-2,5-dione
+
FMNH2
+
O2
=
(+)-5-oxo-1,2-campholide
+
FMN
+
H2O

Synonyms

2,5-diketocamphane 1,2-monooxygenase, 2,5-diketocamphane lactonizing enzyme, 2,5-diketocamphane monooxygenase, 2,5-DKCMO, 2,5-DKCMO-1, 2,5-DKCMO-2, 3,6-diketocamphane 1,6-monooxygenase, camE25-1, camE25-2, camphor ketolactonase I, EC 1.14.13.162, EC 1.14.15.2, ketolactonase I, More, oxygenase, camphor 1,2-mono

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.108 2,5-diketocamphane 1,2-monooxygenase

Specific Activity

Specific Activity on EC 1.14.14.108 - 2,5-diketocamphane 1,2-monooxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.335
-
substrate (+)-camphor
0.43
-
substrate (+)-camphor, purified, reconstituted multimeric enzyme complex
0.81
-
substrate diketocamphane, purified enzyme
1.67
-
component E2, lactonizing
10 - 20
-
component E3, ketolactonizing
24
-
component E2
3.56
-
oxygenating component
38.3
-
component E1, FMN-reductase
417
-
purified component E1 NADH-dehydrogenase
420
-
purified component E1, FMN-coupled NADH-dehydrogenase
additional information
-
no enzyme assay possible in crude extract due to competition with other enzymes for NADH