1.14.14.106: taxane 13alpha-hydroxylase

This is an abbreviated version!
For detailed information about taxane 13alpha-hydroxylase, go to the full flat file.

Reaction

Synonyms

cytochrome P450 taxoid 13alpha-hydroxylase, EC 1.14.13.77, taxoid 13alpha-hydroxylase, THY13a

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.106 taxane 13alpha-hydroxylase

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Results	in table
20	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
2	Cofactor
4	KM Value [mM]
3	Localization
1	Metals/Ions
2	Natural Substrates/ Products (Substrates)
4	Organism
6	Pathway
4	pH Optimum
1	pH Range
1	Purification (Commentary)
1	Reaction
3	Reaction Type
4	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
8	Substrates and Products (Substrate)
5	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.14.14.106 - taxane 13alpha-hydroxylase

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expression of the VSVG-tagged enzyme in Saccharomyces cerevisiae YPH499 cells, expression of the enzymes from and reconstruction of the taxol biosynthesis pathway 5 early steps by expression of the participating enzymes in yeast, overview

Taxus sp.

672704

functional co-expression with taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli

Taxus cuspidata

672699

heterologous expression in Sf9 insect cells

Taxus cuspidata

Q8W4T9

440249