1.13.11.9: 2,5-dihydroxypyridine 5,6-dioxygenase
This is an abbreviated version!
For detailed information about 2,5-dihydroxypyridine 5,6-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.9
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1.13.11.9
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putida
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copperi
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dopamine
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deformylase
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alpha-hydroxylating
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peptidylglycine
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beta-monooxygenase
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biotechnology
- 1.13.11.9
- putida
-
copperi
- dopamine
-
deformylase
-
alpha-hydroxylating
- peptidylglycine
-
beta-monooxygenase
- biotechnology
Reaction
Synonyms
2,5-DHP dioxygenase, 2,5-dihydroxypyridine dioxygenase, 2,5-dihydroxypyridine oxygenase, 2,5DHP dioxygenase, EC 1.13.1.9, NicX, NicX protein, oxygenase, 2,5-dihydroxypyridine 5,6-di-, pyridine-2,5-diol dioxygenase
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Purification
Purification on EC 1.13.11.9 - 2,5-dihydroxypyridine 5,6-dioxygenase
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Escherichia coli cells overexpressing NicX are harvested and disrupted by passage through a French press, supernatant applied to DEAE-cellulose column with 50 mM NaH2PO4 buffer, pH 7.5, washed with buffer (0.1-0.3 M) containing 0.1 M NaCl, fraction with 2,5-dihydroxypyridine deoxygenase activity pooled and dialyzed with 20 mM NaH2PO4 buffer, pH 7.5, loaded onto hydroxyapatite column, eluted with 20-100 mM NaH2PO4 buffer, pH 7.0, fractions with enzyme activity pooled, dialyzed in 20 mM NaH2PO4 buffer, pH 7.5, and concentrated
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recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography
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