Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
General Stability | Organism |
---|---|
combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C | Pseudomonas putida |
dithiothreitol stabilizes | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
2,2'-dipyridyl | - |
Pseudomonas putida | |
EDTA | - |
Pseudomonas putida | |
H2O2 | - |
Pseudomonas putida | |
iodoacetamide | - |
Pseudomonas putida | |
KCN | - |
Pseudomonas putida | |
N-ethylmaleimide | - |
Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida | |
Tiron | i.e. 4,5-dihydroxy-1,3-benzene disulfonic acid | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ requirement | Pseudomonas putida | |
Iron | enzyme contains loosly bound Fe3+ | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
x * 39500, SDS-PAGE | Pseudomonas putida |
242000 | - |
sucrose density gradient centrifugation (+ dithiothreitol) | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxypyridine + O2 + H2O | Pseudomonas putida | nicotinic acid catabolism | maleamate + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-40°C, 3 months, 20% loss of activity | Pseudomonas putida |
0°C, 20 mM sodium phosphate, pH 7.5, half-life: 2-3 days, addition of dithiothreitol extends half-life to about 2 weeks | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxypyridine + O2 + H2O | strictly specific for 2,5-dihydroxypyridine | Pseudomonas putida | maleamate + formate | - |
? | |
2,5-dihydroxypyridine + O2 + H2O | nicotinic acid catabolism | Pseudomonas putida | maleamate + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39500, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |