EC Number |
Protein Variants |
Reference |
---|
2.4.1.155 | A592G |
increased specific activity |
638555 |
2.4.1.155 | L188R |
site-directed mutagenesis, inactive mutant |
736192 |
2.4.1.155 | more |
enzyme downregulation in cancer cells by specific shRNA expression |
735590 |
2.4.1.155 | more |
generation of a stable BEL7404 Tet-on-Mgat5 cell line in which human Mgat5 can be specifically induced by doxycycline to explore functional alterations after Mgat5 overexpression |
736192 |
2.4.1.155 | more |
generation of cell line Lec4A lacking N-acetylglucosaminyltransferase V activity and protein in the Golgi apparatus |
736218 |
2.4.1.155 | more |
GlcNAc and tet-induced Mgat5 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions and complex-type N-glycan branching in HEK293 Mgat5 clones. Up-regulation of Mgat5 in HEK293 cells stimulates amino acid uptake and increases metabolite levels under low Gln/Glc culture conditions |
736200 |
2.4.1.155 | more |
GnT-V enzyme silencing by siRNA in Huh-7 and HepG2 cells |
759648 |
2.4.1.155 | more |
GnT-V is used as a molecular target to further sensitize cetuximab-treated nasopharyngeal carcinoma (NPC) cells to radiation. Development of GnT-V-suppressed cell models, denoted CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224. Lentivirus-mediated shRNA inhibits GnT-V mRNA and protein expression in NPC cell lines. The expression levels of GnTV-V mRNA in these cell models were 36.3%, 33.9%, 42% and 35.5% of the levels found in the control groups, respectively. Similarly, the GnT-V protein levels in the CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224 cell models are decreased by 60.13%, 59.89%, 43.67% and 46.18%, respectively. GnT-V expression and depletion of GnT-V in nasopharyngeal carcinoma cells, phenotypes, overview. Changes in the radiosensitivity of CNE-1 and CNE-2 cells are also related to PI3K/Akt signaling. The total Akt level does not vary among the different groups, but the phospho-Akt and phospho-PI3K levels present differences: the phospho-Akt and phospho-PI3K levels in CNE2-1564 and CNE2-2224 cells are lower than those in CNE-2-NC, and the levels detected in CNE2-1564/cetuximab and CNE2-2224/cetuximab are lower than those found in CNE2-1564 and CNE2-2224 cells. CNE2-NC/cetuximab cells present reduced levels of phospho-Akt and phospho-PI3K compared with CNE2-NC cells. The CNE-1 cells present similar results. In brief, the irradiation-induced alteration of beta-1,6 branches on the EGFR might influence PI3K/AKT signaling |
759306 |
2.4.1.155 | more |
GnT-V overexpression induces an aberrant E-cadherin cellular localization and alters cell morphology, GnT-V increases the stability of the cadherin/catenin complex, GnT-V leads to a reduced intercellular adhesion of gastric cells, phenotype, overview |
721738 |
2.4.1.155 | more |
MGAT5 gene knockout in HT-29 cells using CRISPR/Cas9 |
759884 |