Cloned (Comment) | Organism |
---|---|
gene MGAT5, quantitative real-time PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GnT-V enzyme silencing by siRNA in Huh-7 and HepG2 cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q09328 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
hepatoma cell | - |
Homo sapiens | - |
Huh-7 cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
GnT-V | - |
Homo sapiens |
mannoside acetylglucosaminyltransferase 5 | - |
Homo sapiens |
Mgat5 | - |
Homo sapiens |
N-acetylglucosaminyltransferase V | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | the PI3K/Akt signaling pathway is involved in regulation of GnT-V expression | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | interruption of beta1,6-GlcNAc glycan modification of CD147/basigin decreases matrix metalloproteinase (MMP) expression in HCC cell lines and affects the interaction of CD147/basigin with integrin beta1, mechanism modeling, overview. Real-time PCR shows that MMP-1, MMP-2, and MMP-9 are reduced in cells treated with mutant CD147/basigin (defective beta1,6-branched N-glycosylation) compared with cells treated with wild-type, suggesting that GnT-V-mediated glycosylation increases CD147/basigin-mediated HCC cell invasion. Overexpression of GnT-V increases the level of CD147/basigin-beta1,6-branching and the induction of MMPs | Homo sapiens |
metabolism | activation of the PI3K/Akt pathway is involved in the regulation of GnT-V expression and deletion of GnT-V-mediated N-glycosylation impairs the PI3K/Akt pathway | Homo sapiens |
physiological function | alterations in glycosylation patterns regulate cancer development and progression, serve as important biomarkers, and provide a set of specific targets for diagnosis and therapeutic intervention. The modifications most often associated with cancer include sialylation, beta1,6-GlcNAc-branched N-glycans, and core fucosylation. Increased GlcNAc-branched N-glycan levels result from increased activity of N-acetylglucosaminyltransferase V (GnT-V) encoded by the mannoside acetylglucosaminyltransferase 5 (MGAT5) gene and are closely associated with cancer metastasis. N-acetylglucosaminyltransferase V (GnT-V) regulates cancer processes and cancer cell migration. GnT-V-mediated N-glycosylation of CD147/basigin, a tumor-associated glycoprotein that carries beta1,6-N-acetylglucosamine (beta1,6-GlcNAc) glycans, is upregulated during TGF-beta1-induced epithelial-to-mesenchymal transition (EMT), which correlates with tumor metastasis in patients with hepatocellular carcinoma (HCC). The PI3K/Akt signaling pathway is involved in the regulation of GnT-V expression | Homo sapiens |