EC Number   |
Protein Variants |
Reference |
|---|
    1.18.6.1 | A175G |
shows in vivo 55% of enzyme activity compared to wild-type, in vitro 20% activity remaining with purified enzyme, slowlier conformational change upon binding of MgATP, model of steric interactions using x-ray crystal structures |
440182 |
| 1.18.6.1 | A175S |
unable to support substrate reduction because of an inability to undergo a required MgATP-induced conformational change |
440182 |
| 1.18.6.1 | D125E |
site-directed mutagenesis, mutation alters the properties of the MgATP2- binding site with bound MgADP |
658483 |
| 1.18.6.1 | DELTAC153 |
mutation in the MoFe protein of nitrogenase. The rate of oxidation of Fe-protein F1+ to this MoFe protein variant is unchanged from the rate to the wild-type MoFe protein, providing further evidence against a gated hopping electron tansfer model |
-, 726970 |
| 1.18.6.1 | F388 A |
site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview |
713837 |
| 1.18.6.1 | F388H |
site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview |
713837 |
| 1.18.6.1 | F388T |
site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview |
713837 |
| 1.18.6.1 | F388Y |
site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview |
713837 |
| 1.18.6.1 | G69S |
random mutagenesis, beta-subunit residue mutant of the MoFe protein shows highly decreased affinity for acetylene, acetylene inhibits the mutants nitrogen reduction activity in a competitive mode in contrast to the wild-type enzyme |
658483 |
| 1.18.6.1 | H195G |
alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, slightly decreased Fe protein activity, altered phenotype |
440189 |
