EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.2.1.5 | 0.003 |
- |
acetaldehyde |
with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C |
725948 |
1.2.1.5 | 0.09 |
- |
Isovaleraldehyde |
with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C |
725948 |
1.2.1.5 | 0.11 |
- |
D-glyceraldehyde |
pH 8, 80°C |
722210 |
1.2.1.5 | 0.14 |
- |
D-glyceraldehyde 3-phosphate |
pH 8, 80°C |
722210 |
1.2.1.5 | 0.16 |
- |
NAD+ |
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C |
739913 |
1.2.1.5 | 0.18 |
- |
acetaldehyde |
pH 8, 80°C |
722210 |
1.2.1.5 | 0.24 |
- |
acetaldehyde |
with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C |
725948 |
1.2.1.5 | 0.36 |
- |
Isovaleraldehyde |
with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C |
725948 |
1.2.1.5 | 0.42 |
- |
NAD+ |
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C |
739913 |
1.2.1.5 | 0.5 |
- |
NAD+ |
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C |
739913 |