Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 500 mM, 1.31fold activation | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
General Stability | Organism |
---|---|
the enzyme is stable to 50 mM PMSF | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10% (v/v), 55% inhibition | Sulfurisphaera tokodaii | |
acetone | 10% (v/v), 21% inhibition | Sulfurisphaera tokodaii | |
DMSO | 10% (v/v), 32% inhibition | Sulfurisphaera tokodaii | |
EDTA | 50 mM, 22% inhibition | Sulfurisphaera tokodaii | |
ethanediol | 10% (v/v), 60% inhibition | Sulfurisphaera tokodaii | |
guanidine hydrochloride | 10% (v/v), 42% inhibition | Sulfurisphaera tokodaii | |
H2O2 | the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol | Sulfurisphaera tokodaii | |
nitroglycerin | more than half of the original activity is retained in the presence of 5.5 mM nitroglycerin and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin. Dithiothreitol and alpha-lipoic acid can also restore the nitroglycerin inactivated dehydrogenation to more than half of the original activity | Sulfurisphaera tokodaii | |
PEG-400 | 10% (v/v), 16% inhibition | Sulfurisphaera tokodaii | |
PMSF | 50 mM, no loss of activity | Sulfurisphaera tokodaii | |
Urea | 500 mM, 44% inhibition | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
3.81 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
6.12 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
6.38 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
8.51 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 500 mM, 1.3fold activation | Sulfurisphaera tokodaii | |
Li+ | 500 mM, 107% of the activity compared to activity without metal ions | Sulfurisphaera tokodaii | |
Mg2+ | 20 mM, 107% of the activity compared to activity without metal ions | Sulfurisphaera tokodaii | |
Na+ | 500 mM, 1.2fold activation | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
51300 | - |
4 * 51300, calculation from sequence | Sulfurisphaera tokodaii |
200000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organic Solvent | Comment | Organism |
---|---|---|
2-mercaptoethanol | 10% (v/v), 55% loss of activity | Sulfurisphaera tokodaii |
Acetone | 10% (v/v), 21% loss of activity | Sulfurisphaera tokodaii |
DMSO | 10% (v/v),% loss of activity | Sulfurisphaera tokodaii |
Ethanol | 10% (v/v), 2% loss of activity | Sulfurisphaera tokodaii |
Ethylene glycol | 10% (v/v), 60% loss of activity | Sulfurisphaera tokodaii |
guanidine-HCl | 10% (v/v), 42% loss of activity | Sulfurisphaera tokodaii |
isopropanol | 10% (v/v), 8% loss of activity | Sulfurisphaera tokodaii |
Methanol | 10% (v/v), 7% loss of activity | Sulfurisphaera tokodaii |
polyethyleneglycol | PEG-400, 10% (v/v), 16% loss of activity | Sulfurisphaera tokodaii |
SDS | 10% (v/v), 3% loss of activity | Sulfurisphaera tokodaii |
urea | 500 mM, 44% loss of activity | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q976X5 | - |
- |
Sulfurisphaera tokodaii 7 | Q976X5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dihydroxyacetone + NAD+ + H2O | 53.93% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | ? + NADH + H+ | - |
? | |
3-nitrobenzaldehyde + NAD+ + H2O | 32.98% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | 3-nitrobenzoate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ | Sulfurisphaera tokodaii | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ | Sulfurisphaera tokodaii 7 | acetate + NADH + H+ | - |
? | |
acetaldehyde + NADP+ + H2O | - |
Sulfurisphaera tokodaii | acetate + NADPH | - |
? | |
benzaldehyde + NAD+ + H2O | 35.5% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 35.5% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | benzoate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 48.15% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | butyrate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 48.15% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | butyrate + NADH + H+ | - |
? | |
D-glyceraldehyde + NAD+ + H2O | 50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii | D-glycerate + NADH + H+ | - |
? | |
D-glyceraldehyde + NADP+ + H2O | 50.25% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate + NADPH | - |
? | |
D-glyceraldehyde 3-phosphate + NAD+ + H2O | 52.7% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate 3-phosphate + NADH + H+ | - |
? | |
glycolaldehyde + NAD+ + H2O | 75.43% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | glycolate + NADH + H+ | - |
? | |
additional information | no activity with methanol or ethanol | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | no activity with methanol or ethanol | Sulfurisphaera tokodaii 7 | ? | - |
? | |
propionaldehyde + NAD+ + H2O | 56.22% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii | propionate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | 56.22% of the activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | propionate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
tetramer | 4 * 51300, calculation from sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ST0064 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | 70°C: about 50% of maximal activity, 90°C: about 90% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
coenzyme NAD+ or NADP+ can regulate the enzymatic thermostability | Sulfurisphaera tokodaii |
70 | 80 | about 20% increase of activity after treatment at 70 and 80°C for 2 h, probably due to thermal activation of the enzyme | Sulfurisphaera tokodaii |
90 | - |
half-life: 4 h. Significant protection against thermal inactivation by 1 mM NAD+ or NADP+ when the preheating time is above 4 h at 90°C | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
0.92 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
1.14 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
2.05 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
3.71 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii | |
NADP+ | kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
D-glyceraldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
0.14 | - |
D-glyceraldehyde 3-phosphate | pH 8, 80°C | Sulfurisphaera tokodaii | |
0.18 | - |
acetaldehyde | pH 8, 80°C | Sulfurisphaera tokodaii | |
0.98 | - |
NADP+ | pH 8, 80°C | Sulfurisphaera tokodaii | |
2.45 | - |
NAD+ | pH 8, 80°C | Sulfurisphaera tokodaii |