Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacter brockii subsp. brockii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using either 10% (w/v) PEG 4000, 0.1 M Tris-HCl pH 7.5, and 1% (v/v) 2-propanol or 10% (w/v) PEG 4000, 0.1 M Tris-HCl pH 7.5, and 1% (w/v) n-octyl-beta-D-glucoside | Thermoanaerobacter brockii subsp. brockii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84995 | - |
2 * 84995, calculated from amino acid sequence | Thermoanaerobacter brockii subsp. brockii |
90000 | - |
2 * 90000, SDS-PAGE | Thermoanaerobacter brockii subsp. brockii |
180000 | - |
gel filtration | Thermoanaerobacter brockii subsp. brockii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter brockii subsp. brockii | - |
- |
- |
Thermoanaerobacter brockii subsp. brockii ATCC 33075 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex 200 gel filtration | Thermoanaerobacter brockii subsp. brockii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | - |
Thermoanaerobacter brockii subsp. brockii | beta-D-glucose 1-phosphate + D-glucose | - |
r | |
alpha,alpha-trehalose + phosphate | - |
Thermoanaerobacter brockii subsp. brockii ATCC 33075 | beta-D-glucose 1-phosphate + D-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 90000, SDS-PAGE | Thermoanaerobacter brockii subsp. brockii |
homodimer | 2 * 84995, calculated from amino acid sequence | Thermoanaerobacter brockii subsp. brockii |
Synonyms | Comment | Organism |
---|---|---|
trehalose phosphorylase | - |
Thermoanaerobacter brockii subsp. brockii |