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6-deoxy-D-glucose + alpha-D-glucose 1-phosphate
alpha-D-glucopyranosyl 6-deoxy-alpha-D-glucopyranoside + phosphate
-
at 93% the activity with rate of glucose glucosylation
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate
D-glucose + beta-D-galactose 1-phosphate
-
while the wild type enzyme shows very low activity, the mutant enzyme L649G/A693Q/W371Y displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product
-
-
r
beta-D-glucose 1-phosphate + alpha-D-allose
?
-
4.5% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-arabinose
?
-
1.8% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-fucose
?
-
22% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-galactose
?
-
83.7% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-lyxose
?
-
1.3% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-mannose
?
-
14.1% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-xylose
?
-
135.2% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-L-fucose
?
-
109.2% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + beta-L-arabinose
?
-
79.9% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + D-galactose
? + phosphate
low activity. The Km value for D-galactose is about 30fold higher than that for D-glucose
-
-
r
beta-D-glucose 1-phosphate + D-glucose
alpha,alpha-trehalose + phosphate
-
100% activity with D-glucose
-
-
r
beta-D-glucose-1-phosphate + gentiobiose
6-O-beta-D-glucopyranosyl trehalose + phosphate
-
-
-
-
?
beta-D-glucose-1-phosphate + isomaltose
6-O-alpha-D-glucopyranosyl trehalose + phosphate
-
-
-
-
?
beta-D-glucose-1-phosphate + isomaltotriose
?
-
-
-
-
?
beta-D-glucose-1-phosphate + isopanose
?
-
-
-
-
?
beta-D-glucose-1-phosphate + melibiose
6-O-alpha-D-galactopyranosyl trehalose + phosphate
-
-
-
-
?
D-galactose + beta-D-glucose 1-phosphate
lactotrehalose + phosphate
-
-
-
-
r
D-glucose + beta-D-galactose 1-phosphate
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate
-
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
lactotrehalose + phosphate
D-galactose + beta-D-glucose 1-phosphate
-
-
-
-
r
xylose + beta-D-glucose 1-phosphate
glucosyl-1,1-xylose 1-phosphate
-
glucosylated at 23% of the rate of glucose glucosylation
-
?
additional information
?
-
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
-
specific substrate
-
-
r
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
enzyme is involved in trehalose catabolism
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
best substrate combination
-
-
r
additional information
?
-
-
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
-
-
?
additional information
?
-
the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose
-
-
?
additional information
?
-
-
the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
D-galactose + beta-D-glucose 1-phosphate
lactotrehalose + phosphate
-
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
lactotrehalose + phosphate
D-galactose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
enzyme is involved in trehalose catabolism
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.86
alpha,alpha-trehalose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
64.8
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
171.5
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
20.3
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
183.4
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.7 - 3.8
beta-D-galactose 1-phosphate
0.7 - 38
beta-D-glucose 1-phosphate
0.7
beta-D-galactose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
3.8
beta-D-galactose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
0.7
beta-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
0.75
beta-D-glucose 1-phosphate
-
-
2
beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
3.1
beta-D-glucose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
6
beta-D-glucose 1-phosphate
-
-
38
beta-D-glucose 1-phosphate
-
-
3 - 3.5
D-galactose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
30.1
D-galactose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
35.1
D-galactose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
38
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
43.7
D-galactose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
43.8
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
45.2
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
45.7
D-galactose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
45.8
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
47.5
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
53.5
D-galactose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
53.6
D-galactose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
56.8
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
58
D-galactose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
58.1
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
61.1
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
61.9
D-galactose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
62.5
D-galactose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
68.2
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
96
D-galactose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
98
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
125
D-galactose
-
wild type enzyme, at pH 6.0 and 60°C
447.6
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
0.6
D-glucose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.6
D-glucose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.7
D-glucose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
1.2
D-glucose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
1.2
D-glucose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
1.3
D-glucose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
1.8
D-glucose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
1.9
D-glucose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
1.9
D-glucose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
2.1
D-glucose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
2.1
D-glucose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
2.2
D-glucose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
2.3
D-glucose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
2.3
D-glucose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
2.5
D-glucose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
3
D-glucose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
3.2
D-glucose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
3.5
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
3.7
D-glucose
-
wild type enzyme, at pH 6.0 and 60°C
4.6
D-glucose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
5.5
D-glucose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
36.8
D-glucose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
23
glucose
-
-
0.14
phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
0.97
trehalose
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
210
alpha,alpha-trehalose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
51.4
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
54
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
64.3
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
68.1
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
1.2 - 1.6
beta-D-galactose 1-phosphate
7.4 - 660
beta-D-glucose 1-phosphate
1.2
beta-D-galactose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
1.6
beta-D-galactose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
7.4
beta-D-glucose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
68
beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
114
beta-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
660
beta-D-glucose 1-phosphate
-
-
0.5
D-galactose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
14
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
17
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
21.1
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
22.6
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
23
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
23.3
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
23.6
D-galactose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
24
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
26.2
D-galactose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
27.7
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
31.9
D-galactose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
32.4
D-galactose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
32.9
D-galactose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
32.9
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
33.3
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
35.5
D-galactose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
38.3
D-galactose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
38.9
D-galactose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
42.2
D-galactose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
54.3
D-galactose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
66.5
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
113
D-galactose
-
wild type enzyme, at pH 6.0 and 60°C
4.2
D-glucose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
7
D-glucose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
16.1
D-glucose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
18.6
D-glucose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
21.7
D-glucose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
25
D-glucose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
26.3
D-glucose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
27.4
D-glucose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
30.6
D-glucose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
31.4
D-glucose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
32.1
D-glucose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
32.7
D-glucose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
33.9
D-glucose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
36.1
D-glucose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
41.2
D-glucose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
42
D-glucose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
42.7
D-glucose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
43.3
D-glucose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
43.5
D-glucose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
46.9
D-glucose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
51.2
D-glucose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
53.9
D-glucose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
59
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
60
D-glucose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
86
D-glucose
-
wild type enzyme, at pH 6.0 and 60°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.793
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.315
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
3.17
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.371
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.149
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
0.336
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
0.337
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
0.368
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.388
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
0.413
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
0.444
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
0.488
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
0.5
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
0.509
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.545
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
0.566
D-galactose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
0.574
D-galactose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
0.6
D-galactose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
0.604
D-galactose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
0.613
D-galactose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
0.672
D-galactose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.72
D-galactose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
0.789
D-galactose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
0.952
D-galactose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
1.29
D-galactose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
0.872
D-glucose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
11
D-glucose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
12
D-glucose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
13
D-glucose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
14
D-glucose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
16
D-glucose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
17
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
17
D-glucose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
18
D-glucose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
18
D-glucose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
18
D-glucose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
18
D-glucose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
19
D-glucose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
20
D-glucose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
20
D-glucose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
22
D-glucose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
22
D-glucose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
24
D-glucose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
27
D-glucose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
28
D-glucose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
29
D-glucose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
36
D-glucose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
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L649G
-
the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme
L649G/A693Q
-
the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme
L649G/A693Q/W371Y
-
the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme but displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product
A431T
the mutant shows strongly decreased catalytic efficiency for D-glucose and decreased catalytic efficiency for D-galactose compared to the wild type enzyme
A440E
the mutant shows increased catalytic efficiency for D-glucose and wild type catalytic efficiency for D-galactose compared to the wild type enzyme
A440K
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
A440M
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
A440V
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
A440V/N657Y
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
A440V/R448S
the mutant shows increased catalytic efficiency for D-glucose and strongly increased catalytic efficiency for D-galactose compared to the wild type enzyme
N657D
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
N657G
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
N657I
the mutant shows approximate 2fold increase in affinity for D-galactose
N657L
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
N657R
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
N657Y
the mutant shows approximate 2fold increase in affinity for D-galactose
P588H
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
R448D
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
R448F
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
R448N
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
R448S
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
R448S/N657Y
the mutant shows about wild type catalytic efficiencies for D-glucose and D-galactose
R448V
the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme
additional information
-
construction of chimeric phosphorylases of the kojibiose phosphorylase gene and the trehalose phosphorylase gene from Thermoanaerobacter brockii
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46
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brenda
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101
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2006
Thermoanaerobacter brockii
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66
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