Cloned (Comment) | Organism |
---|---|
expression of His- and STREP-tagged CESA4, CESA7, and CESA8 in Arabidopsis thaliana using the Agrobacterium tumefaciens transfection nethod in the mutant lines are irx1-1, irx3-1, and irx5-2 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the naturally occuring irx3-1 and irx5-2 mutations are caused by premature stop codons that result in protein truncation of CESA7 and CESA4, respectively. In the naturally occuring irx3-1 background, interaction between CESA4 and CESA8 is greatly reduced, and the proteins fail to localize to the plasma membrane. Cellulose contents of mutant lines irx5 and irx1 plants recombinantly expressing CESA8 and CESA4, irx5-2:STREP-CESA4 and irx1-1:STREPCESA8, are much lower than wild-type and as such are not fully functional. Recombinant mutant irx3-1 plants expressing STREP-CESA7 and His/FLAGCESA7 in a dual tag system have wild-type cellulose content and xylem phenotypes, overview. irx3-1:STREP-CESA7-irx3-1:His/FLAG-CESA7 crossed plants phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | secondary | Arabidopsis thaliana | 5618 | - |
microsome | - |
Arabidopsis thaliana | - |
- |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | cellulose synthase A is a catalytic component of the cellulose synthase complex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
Landsberg erecta ecotype, gene CESA7 | - |
Purification (Comment) | Organism |
---|---|
native oligomers partially from microsomes as fragments of the cellulose synthase complex by dual epitope tagging of the CESA7 protein, selection for CESA multimers, overview. Recombinant His- and STREP-tagged CESA4, CESA7, and CESA8, as well as double mutant irx3-1:STREP-CESA7-irx3-1:His/FLAG-CESA7 from Arabidopsis thaliana irx1-1, irx3-1, and irx5-2 mutant plants by nickel and streptavidin affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cellulose synthase A is a catalytic component of the cellulose synthase complex | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | cellulose synthase A is a catalytic component of the cellulose synthase complex. Mass spectrometric analysis of purified cellulose synthase components, His- and STREP-tagged CESA4, CESA7, and CESA8 from irx1-1, irx3-1, and irx5-2 mutant plants, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cellulose synthase A | - |
Arabidopsis thaliana |
cellulose synthase complex | - |
Arabidopsis thaliana |
CesA | - |
Arabidopsis thaliana |
CSC | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the naturally occuring irx3-1 and irx5-2 mutations are caused by premature stop codons that result in protein truncation of CESA7 and CESA4,respectively. In the naturally occuring irx3-1 background, interaction between CESA4 and CESA8 is greatly reduced, and the proteins fail to localize to the plasma membrane | Arabidopsis thaliana |