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Literature summary for 2.3.1.309 extracted from

  • Chu, C.; Hou, F.; Zhang, J.; Phu, L.; Loktev, A.; Kirkpatrick, D.; Jackson, P.; Zhao, Y.; Zou, H.
    A novel acetylation of beta-tubulin by San modulates microtubule polymerization via down-regulating tubulin incorporation (2011), Mol. Biol. Cell, 22, 448-456 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 (DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
K252A acetylation of K252A mutants is reduced to approximately 30% of that of the wild type enzyme. In approximately 50% of the transfected cells, this mutant is detected on seemingly normal microtubules. In the rest of the cells, the microtubule cytoskeleton becomes disorganized and/or disintegrated Homo sapiens
K252Q beta-tubulin is indistinguishable from that expressed in the wild-type control Homo sapiens
K324R the K324R mutation does not significantly reduce the level of acetylation. beta-Tubulin is indistinguishable from that expressed in the wild-type control Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + a [beta-tubulin]-L-lysine Homo sapiens the enzyme acetylates L-lysine at position 252 of beta-tubulin CoA + a [beta-tubulin]-N6-acetyl-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + a [beta-tubulin]-L-lysine the enzyme acetylates L-lysine at position 252 of beta-tubulin Homo sapiens CoA + a [beta-tubulin]-N6-acetyl-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
SAN
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction depleting up to 80% of San from HeLa cells significantly increases the rate of microtubule regrowth after microtubule catastrophe, although no gross defect in microtubule cytoskeleton is observed Homo sapiens
physiological function the enzyme acetylates L-lysine at position 252 of beta-tubulin, which is located at the interface of alpha/beta-tubulin heterodimers and interacts with the phosphate group of the alpha-tubulin-bound GTP. The acetylation is thought to attenuate tubulin incorporation into microtubules Homo sapiens