Cloned (Comment) | Organism |
---|---|
expression of ZmCKX1 in Pichia pastoris | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, redox potential of electron acceptors | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(2-isopentenyl)adenine + FAD | Zea mays | the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine | adenine + 3-methylbut-2-enal + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q9T0N8 | enzyme and gene ZmCKX1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor | reaction scheme and mechanism, reduction of N6-(2-isopentenyl)adenine is rapidly performed, the reaction with O2 is slowly | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity with cytokinins, overiew | Zea mays | ? | - |
? | |
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced 1,4-naphthoquinone | - |
? | |
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | i.e. coenzyme Q0 | Zea mays | adenine + 3-methylbut-2-enal + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
? | |
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
N6-(2-isopentenyl)adenine + caffeic acid | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + coenzyme Q1 | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced coenzyme Q1 | - |
? | |
N6-(2-isopentenyl)adenine + cytochrome c | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced cytochrome c | - |
? | |
N6-(2-isopentenyl)adenine + daphnoretin | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + FAD | - |
Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
? | |
N6-(2-isopentenyl)adenine + FAD | the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
? | |
N6-(2-isopentenyl)adenine + o-coumaric acid | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + O2 | oxidative degradation of cytokinins, slow reaction, low substrate specificity | Zea mays | adenine + 3-methylbut-2-enal + H2O2 | - |
? | |
N6-(2-isopentenyl)adenine + plantacyanine | electron acceptor from spinach | Zea mays | adenine + 3-methylbut-2-enal + reduced plantacyanine | - |
? | |
N6-(2-isopentenyl)adenine + rosmarinic acid | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + scopoletin | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + spinach plantacyanine | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced spinach plantacyanine | - |
r | |
N6-(2-isopentenyl)adenine + vitamin K1 | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced vitamin K1 | - |
? | |
N6-(2-isopentenyl)adenine + vitamin K3 | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced vitamin K3 | - |
? | |
N6-(2-isopentenyl)adenosine + FAD | - |
Zea mays | adenosine + 3-methylbut-2-enal + FADH2 | - |
? | |
zeatin + FAD | - |
Zea mays | ? + FADH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CKX | - |
Zea mays |
cytokinin oxidase/dehydrogenase | - |
Zea mays |
More | enzyme belongs to the plant CKX family | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | at pH above pH 6.5 polymerization of the quinone acceptors occur | Zea mays | |
0.1 | - |
O2 | below, pH 8.0, recombinant enzyme | Zea mays | |
0.3 | - |
caffeic acid | pH 6.5, recombinant enzyme | Zea mays | |
0.3 | - |
o-coumaric acid | pH 6.5, recombinant enzyme | Zea mays | |
0.4 | - |
rosmarinic acid | pH 6.5, recombinant enzyme | Zea mays | |
0.6 | - |
O2 | pH 6.5, recombinant enzyme | Zea mays | |
0.8 | - |
cytochrome c | pH 6.5, recombinant enzyme | Zea mays | |
0.9 | - |
spinach plantacyanine | pH 6.5, recombinant enzyme | Zea mays | |
1 | - |
daphnoretin | pH 6.5, recombinant enzyme | Zea mays | |
1.4 | - |
scopoletin | pH 6.5, recombinant enzyme | Zea mays | |
2.3 | - |
vitamin K1 | pH 6.5, recombinant enzyme | Zea mays | |
85.4 | - |
vitamin K3 | pH 6.5, recombinant enzyme | Zea mays | |
123.1 | - |
coenzyme Q1 | pH 6.5, recombinant enzyme | Zea mays | |
129.8 | - |
N6-(2-isopentenyl)adenosine | pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone | Zea mays | |
143.6 | - |
2,3-dimethoxy-5-methyl-1,4-benzoquinone | pH 6.5, recombinant enzyme | Zea mays | |
143.6 | - |
N6-(2-isopentenyl)adenine | pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone | Zea mays | |
169.6 | - |
1,4-Naphthoquinone | pH 6.5, recombinant enzyme | Zea mays | |
178.4 | - |
2,6-dichlorophenolindophenol | pH 6.5, recombinant enzyme | Zea mays | |
278 | - |
2,6-dichlorophenolindophenol | pH 8.0, recombinant enzyme | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
substrate-dependent pH-profile, overview | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound, redox potential analysis | Zea mays | |
additional information | enzyme shows a preference for quinones as electron acceptors | Zea mays |