Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | - |
Oryza sativa | |
arsenate | dramatically increases expression of ACR2.1 | Oryza sativa | |
additional information | ACR2.1 exhibits higher reductase activity than ACR2.2 | Oryza sativa | |
additional information | ACR2.1 exhibits higher reductase activity than ACR2.2. Expression of ACR2.1 under conditions of phosphate starvation is faster than in the presence of phosphate | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
expressed in an Escherichia coli strain in which the arsC gene is deleted and in a yeast (Saccharomyces cerevisiae) strain with a disrupted ACR2 gene | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
C71S | mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
reduced glutaredoxin | - |
Oryza sativa | |
0.2 | - |
reduced glutaredoxin | - |
Oryza sativa | |
9.2 | - |
arsenate | - |
Oryza sativa | |
9.9 | - |
arsenate | mutant C71S | Oryza sativa | |
12.2 | - |
arsenate | - |
Oryza sativa | |
24.4 | - |
arsenate | mutant C71S | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14330 | - |
sequence analysis | Oryza sativa |
14960 | - |
sequence analysis | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q10SX6 | - |
- |
Oryza sativa | Q336V5 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel-nitrilotriacetic acid chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | ACR2.2 is observed only in roots following arsenate exposure | Oryza sativa | - |
root | weak expression of ACR2.1 before addition of arsenate | Oryza sativa | - |
shoot | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arsenate + reduced glutaredoxin | yeast glutaredoxin ScGrx1p or Escherichia coli Grx2 | Oryza sativa | arsenite + acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACR2.1 | - |
Oryza sativa |
ACR2.2 | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
arsenate | mutant C70S | Oryza sativa | |
0.05 | - |
arsenate | mutant C71S | Oryza sativa | |
0.14 | - |
arsenate | - |
Oryza sativa | |
0.19 | - |
reduced glutaredoxin | - |
Oryza sativa | |
0.26 | - |
reduced glutaredoxin | - |
Oryza sativa | |
0.33 | - |
arsenate | - |
Oryza sativa |