Cloned (Comment) | Organism |
---|---|
expression of thec-myc-tagged enzyme mutant Y137F in Pichia pastoris membranes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
brodifacoum | - |
Rattus norvegicus | |
bromadiolone | - |
Rattus norvegicus | |
chlorophacinone | - |
Rattus norvegicus | |
Difenacoum | - |
Rattus norvegicus | |
difethialone | - |
Rattus norvegicus | |
additional information | VKORC1 mutant Y137F is resistant against inhibition by warfarin, but not against inhibition by other antocoagulants, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-phytyl-1,4-naphthoquinone + oxidized dithiothreitol + H2O | Rattus norvegicus | - |
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
gene Vkorc1, VKORC1 mutant Y137F | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-phytyl-1,4-naphthoquinone + oxidized dithiothreitol + H2O | - |
Rattus norvegicus | 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
mutant Y137F, pH 7.4, 37°C | Rattus norvegicus | difethialone | |
0.00007 | - |
mutant Y137F, pH 7.4, 37°C | Rattus norvegicus | brodifacoum | |
0.0001 | - |
mutant Y137F, pH 7.4, 37°C | Rattus norvegicus | Difenacoum | |
0.00061 | - |
mutant Y137F, pH 7.4, 37°C | Rattus norvegicus | bromadiolone | |
0.0016 | - |
mutant Y137F, pH 7.4, 37°C | Rattus norvegicus | chlorophacinone |