Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Helianthus annuus |
the open reading frame is cloned behind the constitutive ADH1 promotor of the yeast expression vector pVT-U-102 and transformed in Saccharomyces cerevisiae INVSc1 | Helianthus annuus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Helianthus annuus | - |
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | 7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide | ? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | Helianthus annuus | - |
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | - |
? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | Helianthus annuus | - |
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | (2) | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
- |
- |
Helianthus annuus | Q43469 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Helianthus annuus | (4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | 7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide | ? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Helianthus annuus | (4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | - |
? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms | Helianthus annuus | (4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | - |
? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Helianthus annuus | (4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | (2) | ? | |
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms | Helianthus annuus | (4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O | (2) | ? |
Synonyms | Comment | Organism |
---|---|---|
DELTA8-sphingolipid desaturase | - |
Helianthus annuus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Helianthus annuus |