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(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
fluorinated phytosphinganine + ferrocytochrome b5 + O2 + H+
?
-
the presence of a fluorine atom at the position of the initial hydrogen removal C8-HR leads to a complete suppression of the desaturation reaction, while replacement of C8-HS with fluorine generates a mixture of mainly (Z)- and trace amounts of (E)-fluoroolefine
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
sphinganine + ferrocytochrome b5 + O2 + H+
8-sphingenine + ferricytochrome b5 + H2O
-
-
-
?
sphinganine ceramide + ferrocytochrome b5 + O2 + H+
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
additional information
?
-
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
39.8% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
7.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
the enzyme exhibits preference for (Z)-isomer formation
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
(2)
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
(2)
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
no activity with palmitoleic acid, linoleic acid, and alpha-linolenic acid
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine
-
-
?
additional information
?
-
-
isozyme PtD8A preferably forms (8E)-products, ratio 8(Z):8(E) is 0.25
-
-
?
additional information
?
-
-
isozyme PtD8B preferably forms (8E)-products, ratio 8(Z):8(E) is 0.17
-
-
?
additional information
?
-
-
isozyme PtD8C preferably forms (8Z)-products, ratio 8(Z):8(E) is 12.71
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
-
the enzyme has no DELTA6-desaturase activity
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
(2)
-
?
sphinganine + ferrocytochrome b5 + O2 + H+
8-sphingenine + ferricytochrome b5 + H2O
-
-
-
?
sphinganine ceramide + ferrocytochrome b5 + O2 + H+
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
additional information
?
-
-
the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
39.8% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
7.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
the enzyme exhibits preference for (Z)-isomer formation
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
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expressed as chimera protein with DELTA6 fatty acid desaturase in Saccharomyces cervisiae
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae and Arabidopsis thaliana
expressed in Saccharomyces cerevisiae BY4741 cells
-
expressed in Saccharomyces cerevisiae strain InvSc1
-
expressed in Saccharomyces cerevisiae strain INVScI
expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase
expressed in Saccharomyces cerevisiae strain w303-1a
expressed in Saccharomyces cerevisiae strain W303-A1
expression of this openreading frame in Saccharomyces cerevisiae results in the formation of DELTA8-trans/cis-phytosphingenines not present in wild-type cells
gene AtD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene AtD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene AvD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene BdD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene BoD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene CpD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene CsD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8A, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1
-
gene PtD8B, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1
-
gene RcD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene SbD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene TaD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene TaD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene VvD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene VvD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
the open reading frame is cloned behind the constitutive ADH1 promotor of the yeast expression vector pVT-U-102 and transformed in Saccharomyces cerevisiae INVSc1
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase
-
expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase
-
expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Sperling, P.; Libisch, B.; Zahringer, U.; Napier, J.A.; Heinz, E.
Functional identification of a DELTA8-sphingolipid desaturase from Borago officinalis
Arch. Biochem. Biophys.
388
293-298
2001
Borago officinalis, Borago officinalis (Q9FR82)
brenda
Libisch, B.; Michaelson, L.V.; Lewis, M.J.; Shewry, P.R.; Napier, J.A.
Chimeras of DELTA6-fatty acid and DELTA8-sphingolipid desaturases
Biochem. Biophys. Res. Commun.
279
779-785
2000
Borago officinalis (Q9FR82)
brenda
Sperling, P.; Blume, A.; Zahringer, U.; Heinz, E.
Further characterization of DELTA8-sphingolipid desaturases from higher plants
Biochem. Soc. Trans.
28
638-641
2000
Arabidopsis thaliana, Brassica napus, Helianthus annuus (Q43469)
brenda
Beckmann Christop, B.C.; Rattke Janin, R.J.; Oldham Neil, O.N.; Sperling Petr, S.P.; Heinz Erns, H.E.; Boland Wilhel, B.W.
Characterization of a DELTA8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers
Angew. Chem. Int. Ed. Engl.
41
2298-2300
2002
Helianthus annuus, Helianthus annuus (Q43469)
brenda
Ryan, P.R.; Liu, Q.; Sperling, P.; Dong, B.; Franke, S.; Delhaize, E.
A higher plant DELTA8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants
Plant Physiol.
144
1968-1977
2007
Stylosanthes hamata (A7Y7E1)
brenda
Oura, T.; Kajiwara, S.
Disruption of the sphingolipid DELTA8-desaturase gene causes a delay in morphological changes in Candida albicans
Microbiology
154
3795-3803
2008
Candida albicans, Candida albicans TUA6
brenda
Habel, A.; Sperling, P.; Bartram, S.; Heinz, E.; Boland, W.
Conformational studies on the DELTA8(E,Z)-sphingolipid desaturase from Helianthus annuus with chiral fluoropalmitic acids as mechanistic probes
J. Org. Chem.
75
4975-4982
2010
Helianthus annuus
brenda
Li, S.F.; Song, L.Y.; Zhang, G.J.; Yin, W.B.; Chen, Y.H.; Wang, R.R.; Hu, Z.M.
Newly identified essential amino acid residues affecting DELTA8-sphingolipid desaturase activity revealed by site-directed mutagenesis
Biochem. Biophys. Res. Commun.
416
165-171
2011
Brassica rapa (G9I7G1)
brenda
Song, L.; Zhang, Y.; Li, S.; Hu, J.; Yin, W.; Chen, Y.; Hao, S.; Wang, B.; Wang, R.; Hu, Z.
Identification of the substrate recognition region in the DELTA6-fatty acid and DELTA8-sphingolipid desaturase by fusion mutagenesis
Planta
239
753-763
2014
Ribes nigrum
brenda
Li, S.; Zhang, G.; Yuan, Y.; Wang, C.; Gao, W.; Deng, C.; Lu, L.; Hu, Z.
Three homologous genes encoding functional DELTA8-sphingolipid desaturase in Populus tomentosa
Genes Genomics
36
293-301
2014
Populus tomentosa
-
brenda
Chen, M.; Markham, J.; Cahoon, E.
Sphingolipid DELTA8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis
Plant J.
69
769-781
2012
Arabidopsis thaliana
brenda
Garcia-Maroto, F.; Garrido-Cardenas, J.A.; Michaelson, L.V.; Napier, J.A.; Alonso, D.L.
Cloning and molecular characterisation of a DELTA8-sphingolipid-desaturase from Nicotiana tabacum closely related to DELTA6-acyl-desaturases
Plant Mol. Biol.
64
241-250
2007
Nicotiana tabacum (A4GK73), Nicotiana tabacum
brenda
Li, S.; Zhang, G.; Yuan, Y.; Wang, C.; Gao, W.; Deng, C.; Lu, L.; Hu, Z.
Three homologous genes encoding functional DELTA8-sphingolipid desaturase in Populus tomentosa
Genes Genomics
36
293-301
2014
Populus tomentosa
-
brenda
Li, S.F.; Zhang, G.J.; Zhang, X.J.; Yuan, J.H.; Deng, C.L.; Hu, Z.M.; Gao, W.J.
Genes encoding DELTA8-sphingolipid desaturase from various plants identification, biochemical functions, and evolution
J. Plant Res.
129
979-987
2016
Cucumis sativus (A0A0A0KA29), Carica papaya (A0A193BIJ7), Ricinus communis (B9RDZ7), Sorghum bicolor (C5XAI0), Vitis vinifera (F6H3N5), Vitis vinifera (F6I678), Brachypodium distachyon (I1IPG5), Glycine max (I1J5P4), Glycine max (I1KXS8), Glycine max (I1MXA6), Prunus persica (M5X146), Prunus persica (M5X679), Prunus persica (M5XCX6), Arabidopsis thaliana (Q3EBF7), Arabidopsis thaliana (Q9ZRP7), Aquilegia vulgaris (Q8LLD7), Borago officinalis (Q9FR82), Triticum aestivum (Q9ZTU8), Triticum aestivum (W5FYY9), Populus tomentosa (W8DT12), Populus tomentosa (W8DT41), Populus tomentosa (W8DTJ8)
brenda