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2.4.1.B71: flavonol-3-O-glucuronosyltransferase

This is an abbreviated version!
For detailed information about flavonol-3-O-glucuronosyltransferase, go to the full flat file.

Reaction

UDP-glucuronate
+
a flavonol
=
UDP
+
a flavonol 3-O-beta-D-glucuronoside

Synonyms

flavonoid 3-O-glucuronosyltransferase, glycosyltransferase 5, UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase, VvGT5

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.B71 flavonol-3-O-glucuronosyltransferase

Engineering

Engineering on EC 2.4.1.B71 - flavonol-3-O-glucuronosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
coexpression of Vitis vinifera glucuronosyltransferase VvGT5 catalyzing the 3-O-specific glucuronic acid conjugation, and Veronica persica flavonoid 7-O-specific glucuronosyltransferase UGT88D8 to produce miquelianin and scutellarin A in Escherichia coli. Both plant UGTs are codon optimized, synthesized, and functionally expressed in Escherichia coli. Both genes are incorporated in single vector multi-monocistronic systems, overexpressing UDP-glucoronic pathway genes to develop two independent systems for regiospecific glycosylation