2.4.1.91: flavonol 3-O-glucosyltransferase

This is an abbreviated version!
For detailed information about flavonol 3-O-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

2,4,5-trichlorophenol detoxifying O-glucosyltransferase, 3GlcT, 3GT, CsUGT78A1, DicGT1, F3GT, FaGT6, FaGT7, flavonoid 3-O-glucosyltransferase, flavonoid 3-O-glycosyltransferase, flavonoid-3-O-glucosyltransferase, flavonol 3-O-glucosyltransferase, flavonol 3-O-GT, glucosyltransferase, uridine diphosphoglucose-flavonol 3-O-, GT-I, GT1, GTI, More, RUGT-10, UDP flavonoid/triterpene GT, UDP glucose-flavonoid 3-O-glucosyltransferase, UDP glucose: flavonoid-3-O-glucosyltransferase, UDP glucose:flavonoid 3-O-glucosyltransferase, UDP-glucose: flavonoid 3-O-glucosyltransferase, UDP-glucose: flavonoid 3-O-glucosyltransferase gene, UDP-glucose:flavonoid 3-O-glucosyltransferase, UDP-glucose:flavonoid 3-O-glycosyltransferase, UDP-glucose:flavonol 3-O-glucosyltransferase, UDPG:flavonoid-3-O-glucosyltransferase, UDPglucose:flavonoid 3-O-glycosyltransferase, UDPglucose:flavonol O3-D-glucosyltransferase, UF3GT, UFGT, UFGT3, UGT706C1, UGT707A3, UGT71G1, UGT78K1, uridine 5'-diphosphate-glucose:flavonoid 3-O-glucosyltransferase, uridine diphosphat-glucose:flavonoid 3-O-glycosyltransferase, VL3GT

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.91 flavonol 3-O-glucosyltransferase

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Results	in table
12	Activating Compound
100	AA Sequence
1	Application
1	CAS Registry Number
19	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
8	Expression
14	General Information
2	General Stability
48	Inhibitors
11	kcat/KM [mM/s]
50	KM Value [mM]
1	Localization
5	Metals/Ions
17	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
39	Organism
15	Pathway
3	PDB ID
18	pH Optimum
2	pH Range
1	pH Stability
4	pI Value
11	Protein Variants
17	Purification (Commentary)
2	Reaction
1	Reaction Type
38	Reference
56	Source Tissue
5	Specific Activity [micromol/min/mg]
4	Storage Stability
186	Substrates and Products (Substrate)
8	Subunits
52	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Stability [°C]
12	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.4.1.91 - flavonol 3-O-glucosyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

wild-type and selenomethionine-labeled enzyme in complex with UDP and UDP-glucose, hanging drop vapour diffusion method, 5 mg/ml protein is mixed with 5 mM UDP-galactose and 5 mM quercetin at a 2:1 v/v ratio, mixed with an equal volume of reservoir solution containing 40% w/v PEG 3350, 0.2 M ammonium acetate, and 0.1 M sodium citrate, pH 5.6, equilibration over the reservoir solution at 20°C, 2-5 days, X-ray diffraction structure determination and analysis at 2.0-2.6 A resolution, molecular docking

Medicago truncatula

Q5IFH7

676413

purified recombinant detagged enzyme bound to UDP-2-fluoroglucose and kaempferol, or with UDP and quercetin, hanging drop method, 4.8 mg/ml protein in 5 mM Tris (2-carboxyethyl) phosphine HCl, 10 mM Tris–Cl, pH 8.3, diluted 1:1 with mother liquor which contains 18–22% polyethylene glycol 10 000, 0.1M Bis-Tris propane, pH 7.0, and 0–0.5% v/v Pluronic F-68, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.2 A resolution

Vitis vinifera

-

673320