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2.4.1.7: sucrose phosphorylase

This is an abbreviated version!
For detailed information about sucrose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.7

Reaction

sucrose
+
phosphate
=
D-fructose
 +
alpha-D-glucose 1-phosphate

Synonyms

1149SPase, 1355SPase, 742SPase, BiSP, disaccharide glucosyltransferase, LmSPase, More, SPase, sucrose glucosyltransferase, sucrose: orthophosphate, alpha-D-glucosyltransferase, sucrose: phosphate alpha-D-glucosyltransferase, unspase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.7 sucrose phosphorylase

Cloned

Cloned on EC 2.4.1.7 - sucrose phosphorylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B
DNA and amino acid sequence determination and analysis, phylogenetic tree
-
expressed in Acetobacter strain G7, enhanced cellulose production in transformed cells
-
expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized
WP_094046414.1
expression in Escherichia coli
expression in Escherichia coli BL-21 Star on large scale
expression in Escherichia coli BL21
expression in Escherichia coli BL21. Expression conditions are optimized. The highest expression efficiency is obtained at an initial cell density of OD600 0.5, with 0.05 mM isopropyl-beta-D-thiogalactoside, followed by shaking at 180 rpm and incubation at 30°C for 15 h
expression in Escherichia coli strain JM109
-
expression in Escherichia coli strain JM109, expression and cell cultivation method optimization, overview
-
expression in Escherihia coli strain DH10B
-
expression of His-tagged enzyme in Escherichia coli DH10B
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B
-
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of Strep-tagged wild-type and mutant enzymes in Escherichia coli Top10 cells
-
expression of the His4-tagged enzyme in Escherichia coli strain DH5alpha
-
gene 1355SPase, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain DH5alpha
gene scrP, phylogenetic analysis, enzyme expression analysis in strain LTH5448
gene sucP, cloning from genomic library, DNA and amino acid sequence determination and analysis, determination of promotor region, expression in Escherichia coli
Q84HQ2
heterologous expression in a food-grade Bacillus subtilis strain. Efficiently secreted into the extracellular medium in the absence of a signal peptide. After culturing the recombinant strain in a 3-l bioreactor, crude enzyme yield and activity reach 7.5 g/l and 5.3 U/ml, respectively
-
overexpressed in Escherichia coli, 30% of total soluble protein, high specific activity
-
recombinnat expression in Escherichia coli
-
sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
unspase, genetic library construction, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged unspase in Escherichia coli strain XL-1 Blue