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0.08 - 0.5
2'-fucosyllactose
0.088 - 2.64
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
1.6
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,3)-beta-DGlcNAcp-O(CH2)7CH3
37°C, pH 7.0
0.184
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,4)-beta-D-GlcNAcp-O-(CH2)8CO2CH3
37°C, pH 7.0
0.022
alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
37°C, pH 7.0
0.032 - 0.35
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
1.2
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol)
-
pH 7.4, 22°C
2.2
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl
-
pH 7.4, 22°C
0.006
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein
-
pH 7.4, 22°C
0.82
Fucalpha(1,2)Galbeta-1-(4-nitrophenol)
-
pH 7.4, 22°C
0.84
Fucalpha(1,2)Galbeta1-benzyl
-
pH 7.4, 22°C
3.7
Fucalpha-1-(4-nitrophenol)
-
pH 7.4, 22°C
3.8
Fucalpha-1-benzyl
-
pH 7.4, 22°C
1.5 - 3.22
L-fucosyl-alpha-1,2-alpha-D-galactosyl-O(CH2)7CH3
0.022 - 0.281
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
0.027 - 0.116
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
2.5
lacto-N-fucopentaose I
-
-
0.8
N-acetyllactosamine
-
-
2.2
O-alpha-L-fucosyl-1,2-galactose
-
-
0.2
octyl 3-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
4
octyl 4-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.565
octyl 6'-amino-6'-deoxy-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.538
octyl 6'-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.4
octyl alpha-L-xylo-hexopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.055
UDP-6-deoxygalactose
-
-
0.023 - 0.78
UDP-alpha-D-galactose
0.0035
UDP-Gal
-
pH 7.4, 22°C
0.285 - 0.34
UDP-N-acetylgalactosamine
additional information
additional information
-
0.08
2'-fucosyllactose
-
-
0.5
2'-fucosyllactose
-
-
0.088
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
wild-type, pH 7.0, 37°C
0.1
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant D302E/D316E, pH 7.0, 37°C
0.101
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant R188K, pH 7.0, 37°C
0.117
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant D302A/D316A, pH 7.0, 37°C
0.19
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant E303C, pH 7.0, 37°C
0.332
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant D302C, pH 7.0, 37°C
0.98
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant E303A, pH 7.0, 37°C
2.64
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
mutant E303D, pH 7.0, 37°C
0.032
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose
0.055
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose
0.2
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc
0.3
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc
0.3
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc
0.35
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose
1.5
L-fucosyl-alpha-1,2-alpha-D-galactosyl-O(CH2)7CH3
-
with UDP-galactose as donor
3.22
L-fucosyl-alpha-1,2-alpha-D-galactosyl-O(CH2)7CH3
-
with UDP-N-acetylgalactosamine as donor
0.022
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
-
0.054
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
with UDP-galactose as donor
0.061
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
with UDP-galactose as donor
0.099
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
-
0.25
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
with UDP-N-acetylgalactosamine as donor
0.281
L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
with UDP-N-acetylgalactosamine as donor
0.027
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
0.106
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
P234S mutant
0.11
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
-
0.116
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
-
0.023
UDP-alpha-D-galactose
-
mutant C80S/C196S, pH 7.0, temperature not specified in the publication
0.027
UDP-alpha-D-galactose
wild-type, pH 7.0, 37°C
0.036
UDP-alpha-D-galactose
mutant E303C, pH 7.0, 37°C
0.045
UDP-alpha-D-galactose
-
deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication
0.053
UDP-alpha-D-galactose
mutant D302C, pH 7.0, 37°C
0.074
UDP-alpha-D-galactose
mutant E303A, pH 7.0, 37°C
0.101
UDP-alpha-D-galactose
mutant R188K, pH 7.0, 37°C
0.112
UDP-alpha-D-galactose
mutant D302E/D316E, pH 7.0, 37°C
0.17
UDP-alpha-D-galactose
mutant D302A/D316A, pH 7.0, 37°C
0.211
UDP-alpha-D-galactose
mutant E303D, pH 7.0, 37°C
0.78
UDP-alpha-D-galactose
-
mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication
0.01
UDP-galactose
-
-
0.027
UDP-galactose
wild-type enzyme
0.033
UDP-galactose
37°C, pH 7.0, wild-type enzyme S185C
0.035
UDP-galactose
-
with L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3 as acceptor
0.036
UDP-galactose
-
in reaction with 2'-fucosyllactose
0.042
UDP-galactose
mutant D262N
0.05
UDP-galactose
-
in reaction with N-acetyllactosamine
0.052
UDP-galactose
mutant F216I
0.053
UDP-galactose
37°C, pH 7.0, wild-type enzyme
0.06
UDP-galactose
-
with L-fucosyl-alpha-1,2-alpha-D-galactosyl-O(CH2)7CH3 as acceptor
0.166
UDP-galactose
37°C, pH 7.0, wild-type enzyme S185N
0.222
UDP-galactose
mutant A268T
4.5
UDP-galactose
P234S mutant
0.023
UDP-GalNAc
-
mutant C80S/C196S, pH 7.0, temperature not specified in the publication
0.045
UDP-GalNAc
-
deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication
0.78
UDP-GalNAc
-
mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication
0.12
UDP-glucose
37°C, pH 7.0, wild-type enzyme S185N
0.152
UDP-glucose
37°C, pH 7.0, wild-type enzyme S185C
0.188
UDP-glucose
37°C, pH 7.0, wild-type enzyme
0.285
UDP-N-acetylgalactosamine
-
-
0.3
UDP-N-acetylgalactosamine
-
with L-fucosyl-alpha-1,2-alpha-D-galactosyl-O(CH2)7CH3 as acceptor
0.34
UDP-N-acetylgalactosamine
-
with L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3 as acceptor
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
kinetics, hyperbolic dependence on UDP-Gal concentration
-
additional information
additional information
-
kinetic mechanism and thermodynamic analysis of GTB using electrospray ionization mass spectrometry, comparison to GTA, EC 2.4.1.40
-