2.4.1.216: trehalose 6-phosphate phosphorylase
This is an abbreviated version!
For detailed information about trehalose 6-phosphate phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.216
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2.4.1.216
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inguinal
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hernia
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mesh
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sheath
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preperitoneal
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lichtenstein
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postoperative
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lactis
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1-phosphate
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phosphorolysis
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maltose
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posterior
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department
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unilateral
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outpatient
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endoscopic
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files
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rectus
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expertise
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enterococcus
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beta-phosphoglucomutase
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ethic
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beta-glucose
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netherlands
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groin
-
high-volume
-
phosphorylases
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re-recurrence
-
expert
-
elective
-
faecalis
-
synthesis
- 2.4.1.216
-
inguinal
-
hernia
-
mesh
-
sheath
-
preperitoneal
-
lichtenstein
-
postoperative
- lactis
- 1-phosphate
-
phosphorolysis
- maltose
-
posterior
-
department
-
unilateral
-
outpatient
-
endoscopic
-
files
-
rectus
-
expertise
-
enterococcus
- beta-phosphoglucomutase
-
ethic
- beta-glucose
-
netherlands
-
groin
-
high-volume
- phosphorylases
-
re-recurrence
-
expert
-
elective
- faecalis
- synthesis
Reaction
Synonyms
TPP, trehalose-6-phosphate phosphatase, TrePP
ECTree
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Application
Application on EC 2.4.1.216 - trehalose 6-phosphate phosphorylase
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synthesis
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efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained