2.3.3.13: 2-isopropylmalate synthase
This is an abbreviated version!
For detailed information about 2-isopropylmalate synthase, go to the full flat file.
Reaction
+
+
=
+
Synonyms
2-isopropylmalate synthase 1, chloroplastic, 2-isopropylmalate synthase 2, chloroplastic, 3-hydroxy-4-methyl-3-carboxy-pentanoate 2-oxo-3-methyl-butanoate lyase (CoA-acetylating), 3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methyl-butyrate lyase (CoA-acetylating), alpha-IPM synthase, Alpha-IPM synthetase, alpha-IPMS, alpha-IPMS-14CR, alpha-IPMS-2CR, Alpha-isopropylmalate synthase, alpha-isopropylmalate synthase I, alpha-isopropylmalate synthase II, alpha-isopropylmalate synthetase, alpha-isopropylmalic synthetase, BatIMS, EC 4.1.3.12, IPM synthase, IPM-synthase, IPMS, IPMS1, IPMS2, IPMS3, isopropylmalate synthase, isopropylmalate synthase 1, isopropylmalate synthetase, LEU4, LEU9, leuA, Mj1195, MtalphaIPMS, MtIPMS, NmeIPMS, Os11g04670, Os12g04440, OsIPMS1, OsIPMS2, ROL17, synthase, 2-isopropylmalate, synthase, alpha-isopropylmalate, TT_C0849
ECTree
KI Value
KI Value on EC 2.3.3.13 - 2-isopropylmalate synthase
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2.5
(S)-alpha-hydroxyisovalerate
pH 7.5, 25°C
0.1 - 0.2
2-Oxo-isohexanoate
0.03
3,3-dibromopyruvate
pH 7.5, 25°C
0.06
3-Bromopyruvate
pH 7.5, 25°C
0.0025
Ca2+
-
pH 7.5, 25°C
0.09
desulfo-CoA
-
with respect to acetyl-CoA
230
K+
-
at high concentration
0.00037
Zn2+
-
pH 7.5, 25°C
additional information
leucine
-
Ki with and without Zn2+ or Mn2+
0.1
2-Oxo-isohexanoate
-
with respect to 2-oxo-3-methylbutanoate
0.2
2-Oxo-isohexanoate
-
with respect to acetyl-CoA
0.07
CoA
-
with respect to acetyl-CoA
0.07
CoA
-
interacting with the product site
0.5
isopropylmalate
-
with respect to 2-oxo-3-methylbutanoate at a substrate concentration of 0.07 mM acetyl-CoA
0.9
isopropylmalate
-
with respect to acetyl-CoA at a substrate concentration of 0.375 mM 2-oxo-3-methylbutanoate
4
isopropylmalate
-
with respect to acetyl-CoA at a substrate concentration of 0.75 mM 2-oxo-3-methylbutanoate
0.00016
L-leucine
pH 7.8, 37°C
0.0023
L-leucine
-
mutant enzyme D563N, pH and temperature not specified in the publication
0.003
L-leucine
-
mutant enzyme D563A, pH and temperature not specified in the publication
0.0077
L-leucine
-
mutant enzyme Q566A, pH and temperature not specified in the publication
0.012
L-leucine
wild-type
0.012
L-leucine
-
wild type enzyme, pH and temperature not specified in the publication
0.017
L-leucine
-
pH and temperature not specified in the publication
0.018
L-leucine
mutant Y169F
0.019
L-leucine
mutant H379A
0.023
L-leucine
-
mutant enzyme H540A, pH and temperature not specified in the publication
0.024
L-leucine
-
mutant enzyme D564A, pH and temperature not specified in the publication
0.051
L-leucine
-
mutant enzyme S560W, pH and temperature not specified in the publication
0.139
L-leucine
mutant E218A
0.155
L-leucine
-
mutant enzyme M559A, pH and temperature not specified in the publication
0.16
L-leucine
-
at pH 7.8 and 37 °C
0.001
leucine
-
pH 7.35, acetyl-CoA varied
0.002
leucine
-
pH 7.6, acetyl-CoA varied
0.0025
leucine
-
pH 8.1, acetyl-CoA varied
0.008
leucine
-
pH 6.8, 0.4 mM acetyl-CoA, 0.1 mM 2-oxo-3-methylbutanoate, wild-type enzyme
0.022
leucine
-
pH 6.8, 0.8 mM acetyl-CoA, 4 mM 2-oxo-3-methylbutanoate, wild-type enzyme
0.1
leucine
-
pH 7.6, 2-oxo-3-methylbutanoate varied
0.1
leucine
-
only isoenzyme I activity
0.2
leucine
-
pH 8.1, 2-oxo-3-methylbutanoate varied
0.2
leucine
-
pH: 7.2, half-maximal inhibition
0.81
leucine
-
competitive, with respect to 2-oxo-3-methylbutanoate
1.2
leucine
-
only isoenzyme II activity
1.3
leucine
-
non-competitive, with respect to acetyl-CoA
3
leucine
-
pH 6.8, 0.4 mM acetyl-CoA, 0.1 mM 2-oxo-3-methylbutanoate, feedback-resistent enzyme
20
leucine
-
pH 6.8, 0.8 mM acetyl-CoA, 4 mM 2-oxo-3-methylbutanoate, feedback-resistent enzyme